REL
2018-06-16
2018-08-23
2018-08-23
CryoEM structure of human LRRC8A
Osamu
Nureki
Department of Biological Sciences, School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyo-ku
Tokyo
Japan
113-0033
Osamu
Nureki
Department of Biological Sciences, School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyo-ku
Tokyo
Japan
113-0033
Kasuya G
Nakane T
Yokoyama T
Shirouzu M
Ishitani R
Nureki O
2.9
10.6019/EMPIAR-10196
EMDB
EMD-6952
Nureki O
Kasuya G
Nakane T
Yokoyama T
Jia Y
Inoue M
Watanabe K
Nakamura R
Nishizawa T
Kusakizako T
Tsutsumi A
Yanagisawa H
Dohmae N
Hattori M
Ichijo H
Yan Z
Kikkawa M
Shirouzu M
Ishitani R
Cryo-EM structures of the human volume-regulated anion channel LRRC8
Nature structural & molecular biology
Nat. Struct. Mol. Biol.
Japan
2018
English
10.1038/s41594-018-0109-6
30127360
Unaligned movies of human LRRC8A
/data
micrographs - multiframe
TIFF
TIFF
5805
40
1
40
UNSIGNED BYTE
7420
1.49
7676
1.49
Movies were collected on Talos Arctica at 0.745 A / super-resolution pixel (that is, 1.49 A / physical pixel). Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2". Seven datasets were combined to yield the structure.
Dose rate (shown on SerialEM) is:
Dataset 0: 1.264 e/A2/frame
Dataset 1, 2, 3: 1.245 e/A2/frame
Dataset 4: 1.260 e/A2/frame
Dataset 5: 1.257 e/A2/frame
Dataset 6: 1.265 e/A2/frame