REL 2019-01-24 2019-02-01 2019-02-01 0000-0002-7420-145X Haruhiko Ehara RIKEN 1-7-22, Suehiro-cho Tsurumi, Yokohama Kanagawa Japan 230-0045 0000-0001-8174-8704 Shun-ichi Sekine RIKEN 1-7-22, Suehiro-cho Tsurumi, Yokohama Kanagawa Japan 230-0045 0000-0001-7412-3722 Hitoshi Kurumizaka The University of Tokyo 1-1-1, Yayoi Bunkyo-ku Tokyo Japan 113-0032 Kujirai T Ehara H Fujino Y Shirouzu M Sekine S Kurumizaka H 1.4 10.6019/EMPIAR-10244 EMDB EMD-6982 Kujirai T Ehara H Fujino Y Shirouzu M Sekine S Kurumizaka H Science (New York, N.Y.) Science 6414 362 595 598 2018 English 10.1126/science.aau9904 30287617

Unaligned movies collected on Talos Arctica. (0.745 A / super-resolution pixel) We have used following MotionCor2 parameters for motion correction: -Bft 150 -PixSize 0.745 -Patch 5 5 -Gain SuperRef_XXXX.mrc -FtBin 2 -Kv 200 -FmDose 1.25 -Iter 10 -Throw 2 -RotGain 1 -FlipGain 2

Unaligned movies of RNA polymerase II transcribing a nucleosome /data/movies micrographs - multiframe TIFF TIFF 2719 40 1 40 UNSIGNED BYTE 7420 0.745 7676 0.745

Unaligned movies in compressed TIFF (collected on 4 sessions). The following parameters were used for MotionCor2: -Bft 150 -PixSize 0.745 -Patch 5 5 -Gain SuperRef_XXXX.mrc -FtBin 2 -Kv 200 -FmDose 1.25 -Iter 10 -Throw 2 -RotGain 1 -FlipGain 2