REL
2019-02-14
2019-02-19
2021-07-02
The 1.54 Å structure of Apoferritin by CRYOARM300 with cold-FEG
Takayuki
Kato
Graduated School of Frontier Biosciences, Osaka university
Osaka
Japan
565-0871
Takayuki
Kato
Graduated School of Frontier Biosciences, Osaka university
Osaka
Japan
565-0871
Kato T
Makino F
Nakane T
Terahara N
Kaneko T
Shimizu Y
Motoki S
Ishikawa I
Yonekura K
Namba K
145.9
10.6019/EMPIAR-10248
EMDB
EMD-9865
Kato T
Makino F
Nakane T
Terahara N
Kaneko T
Shimizu Y
Motoki S
Ishikawa I
Yonekura K
Namba K
CryoTEM with a Cold Field Emission Gun That Moves Structural Biology into a New Stage
Microscopy and Microanalysis
Microsc. Microanal.
United Kingdom
25
998
999
2019
English
10.1017/S1431927619005725
Raw, non aligned micrograph movies in compressed TIFF format. The gain reference can be found in the same directory in MRC format. You can use gain.mrc with 90 degree rotaion and no flip for gain correction.
- Mouse H-chain apoferritin
- JEOL CRYOARM300 with Cold-FEG
- Omega type energy filter with 20 eV slit width
- Data collected by JADAS
- 840 movies
- Pixel size 0.495 Å/pix
- Exposure rate 2.3 e/pix/s
- Fractions 50 frames
- Exp time 10 sec
- Defocus 0.4 -1.2 um
- Attention beam tilt was fluctuated
Unaligned multi-frame micrographs of Apoferritin recorded by CRYOARM300
/data/Movies
micrographs - multiframe
TIFF
TIFF
972
50
UNSIGNED 16 BIT INTEGER
3710
0.495
3838
0.495
Raw, non aligned micrograph movies in compressed TIFF format. The gain reference can be found in the same directory in MRC format. You can use gain.mrc with 90 degree rotaion and no flip for gain correction.