REL 2019-03-18 2019-05-02 2019-05-02 0000-0002-1673-2113 Yigong Shi School of Life Sciences, Tsinghua University Qinghuayuan street Beijing Beijing China 100086 0000-0002-1673-2113 Yigong Shi School of Life Sciences Qinghuayuan Street Beijing Beijing China 100086 Su Q 209.8 10.6019/EMPIAR-10262 EMDB EMD-6991 Su Q Hu F Ge X Lei J Yu S Wang T Zhou Q Mei C Shi Y Science Science China 6406 361 eaat9819 eaat9819 2018 English 10.1126/science.aat9819 30093605

A total of 3761 zero-loss movie stacks were automatically collected using AutoEMation II (developed by J. Lei) in the super-resolution mode with 20-eV slit in energy filter at a nominal magnification of 105,000× with defocus range from −1.0 to −2.0 mm. Each micrograph stack, which contained 32 frames, was exposed for 5.6 s with a total electron dose of ~50 e−/Å2. The stacks were motion corrected using MotionCor2 with a binning factor of 2, resulting in a pixel size of 1.091 Å. Dose weighting was performed concurrently. The defocus values were estimated with Gctf.

Autopicked particles of PKD1/PKD2 complex /data picked particles - multiframe - unprocessed MRCS MRCS 3774 32 32 BIT FLOAT variable variable variable variable

data/Particles/stack_3038_cor_DW.mrcs data/Particles/stack_3038_cor_DW.mrcs data