REL
2019-03-24
2019-06-21
2019-06-21
LAT1-CD98hc bound to HBJ127 Fab and MEM-108 Fab
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo, Bunkyo-ku
Tokyo
Japan
113-0033
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo, Bunkyo-ku
Tokyo
Japan
113-0033
Lee Y
Wiriyasermkul P
Jin C
Quan L
Ohgaki R
Okuda S
Kusakizako T
Nishizawa T
Oda K
Ishitani R
Yokoyama T
Nakane T
Shirouzu M
Endou H
Nagamori S
Kanai Y
Nureki O
969.9
10.6019/EMPIAR-10265
EMDB
EMD-9850
Lee Y
Kusakizako T
Nureki O
Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc
Nature structural & molecular biology
Nat. Struct. Mol. Biol.
2019
English
10.1038/s41594-019-0237-7
31160781
Non-gain-corrected micrograph movies in tiff format. Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2".
Exposure time: 15.6 seconds
Frame: 46 frames
Accumulated exposure: 50.2 e−/A2
Electron flux rate: 7.14 e−/pix/s
Unaligned multi-frame micrographs
/data/Movies
micrographs - multiframe
TIFF
TIFF
1869
40
1
40
UNSIGNED BYTE
7420
0.7625
7676
0.7625
The pixel size (0.7625 A/px) is for super-resolution pixels. The defect file (defect.txt) and the gain reference (gain.mrc) are also uploaded. In UCSF MotionCor2, you have to apply "-RotGain 1 -FlipGain 2". The final set of particles and orientations are stored in particle.star. The total exposure is 50.2 electrons per square angstrom in a 15.6 second exposure.