<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10269" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2019-03-05</depositionDate>
            <releaseDate>2019-06-07</releaseDate>
            <updateDate>2019-06-07</updateDate>
        </keyDates>
        <title>Single particle reconstruction of 52 kDa apo-state streptavidin at 3.3 Angstrom resolution</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9494-8780</authorORCID>
            <firstName>Hong-Wei</firstName>
            <lastName>Wang</lastName>
            <organization type="academic">School of life sciences, Tsinghua University</organization>
            <street>Tsinghua University</street>
            <townOrCity>Beijing</townOrCity>
            <stateOrProvince>Beijing</stateOrProvince>
            <country>China</country>
            <postOrZipCode>100084</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9494-8780</authorORCID>
            <firstName>Hong-Wei</firstName>
            <lastName>Wang</lastName>
            <organization type="academic">School of life sciences, Tsinghua University</organization>
            <street>Tsinghua University</street>
            <townOrCity>Beijing</townOrCity>
            <stateOrProvince>Beijing</stateOrProvince>
            <country>China</country>
            <postOrZipCode>100084</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-9855-1641">Fan X</author>
            <author>Wang J</author>
            <author>Zhang X</author>
            <author>Yang Z</author>
            <author>Zhang JC</author>
            <author>Zhao L</author>
            <author authorORCID="0000-0003-1569-0238">Peng HL</author>
            <author authorORCID="0000-0002-9384-8742">Lei J</author>
            <author authorORCID="0000-0001-9494-8780">Wang HW</author>
        </authorsList>
        <datasetSize units="TB">2.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10269</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-0690</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-9855-1641" order="1">Fan X</author>
                    <author order="2">Wang J</author>
                    <author order="3">Zhang X</author>
                    <author order="4">Yang Z</author>
                    <author order="5">Zhang JC</author>
                    <author order="6">Zhao L</author>
                    <author authorORCID="0000-0003-1569-0238" order="7">Peng HL</author>
                    <author authorORCID="0000-0002-9384-8742" order="8">Lei J</author>
                    <author authorORCID="0000-0001-9494-8780" order="9">Wang HW</author>
                    <title>Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>10</volume>
                    <year>2019</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-019-10368-w</externalReferences>
                    <externalReferences type="pubmed">31160591</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Uncorrected apo-state streptavidin movie stacks, binning 2 from super-resolution stacks.</name>
        <directory>/data/apoSA/movies</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1448</numImagesOrTiltSeries>
        <framesPerImage>32</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>32</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>0.5265</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>0.5265</pixelHeight>
        </dimensions>
        <details>particle_coordinates/run_data_23991_final_refine.star
23991 particles for final 3D reconstruction.

particle_coordinates/After2D_split/After2D_onG_134606_particles.star
After 2D classification, 134,606 particles on selected 749 images were assigned to subset A (GWI) according to the special pattern. 

particle_coordinates/After2D_split/After2D_onICE_77499_particles.star
After 2D classification, 77,499 particles on selected 749 images were assigned to subset B (AWI) according to the special pattern. 

particle_coordinates/After3D_split/after3d_onG_35676.star
After 1st round 3D classification, 35,676 particles on selected 749 images were assigned to subset A (GWI) according to the special pattern. 

particle_coordinates/After3D_split/after3d_onICE_8650.star
After 1st round 3D classification, 8,650 particles on selected 749 images were assigned to subset B (AWI) according to the special pattern.</details>
        <segmentationList/>
        <micrographsFilePattern>data/apoSA/movies/*mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/apoSA/particle_coordinates</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/apoSA/particle_coordinates</pickedParticlesDirectory>
    </imageSet>
</entry>
