REL
2019-07-22
2019-08-30
2019-08-30
Cryo-EM structures of human P4-ATPase flippase
0000-0001-7463-8398
Tomohiro
Nishizawa
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyoku
Tokyo
Japan
113-0033
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyoku
Tokyo
Japan
113-0033
Hiraizumi M
Yamashita K
Nishizawa T
Nureki O
11.0
10.6019/EMPIAR-10303
EMDB
EMD-9931
EMD-9932
EMD-9933
EMD-9934
EMD-9935
EMD-9936
EMD-9937
EMD-9938
EMD-9939
EMD-9940
EMD-9941
EMD-9942
Hiraizumi M
Yamashita K
Nisihzawa T
Nureki O
Cryo-EM structures capture the transport cycle of the P4-ATPase flippase
Science (New York, N.Y.)
Science
2019
English
10.1126/science.aay3353
31416931
Movies were collected on Titan Krios G3i with Gatan K3. The calibrated pixel size for all datasets is 0.83 A. Five datasets were used to yield the structures.
Dataset 0: EMD-9938, EMD-9939, EMD-9940 (E2P class 1,2,3)
Dataset 1: EMD-9941 (E2Pi-PL), EMD-9942 (E1P)
Dataset 2: EMD-9937 (E1P-ADP)
Dataset 3: EMD-9931, EMD-9932, EMD-9933 (E1 class 1,2,3)
Dataset 4: EMD-9935, EMD-9934, EMD-9936 (E1-ATP class 1,2,3)
Dose rate (shown on SerialEM) is ~1 e/A2/frame.
Dataset 0 images are gain-corrected. Gain references for datasets 1-4 are provided, which need to be flipped ('Flip upside down (1)' in RELION).
Unaligned movies for E2P class 1,2,3
/data/Movies/Dataset0
micrographs - multiframe
TIFF
TIFF
6257
62
1
62
UNSIGNED BYTE
5760
0.83
4092
0.83
Images are gain-corrected. Note that we started processing with the nominal pixel size of 0.85 Å (as shown in the tiff header) and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.83 Å.
data/Movies/Dataset0/*.tif
data/Particles/dataset0-all.star
data/Particles/Dataset0
Unaligned movies for E2Pi-PL and E1P
/data/Movies/Dataset1
micrographs - multiframe
TIFF
TIFF
6843
54
1
54
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset1/*.tif
data/Particles/dataset1_*-all.star
data/Particles/Dataset1_*
Unaligned movies for E1P-ADP
/data/Movies/Dataset2
micrographs - multiframe
TIFF
TIFF
4401
54
1
54
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset2/*.tif
data/Particles/dataset2-all.star
data/Particles/Dataset2
Unaligned movies for E1 class1,2,3
/data/Movies/Dataset3
micrographs - multiframe
TIFF
TIFF
3590
54
1
54
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset3/*.tif
data/Particles/dataset3-all.star
data/Particles/Dataset3
Unaligned movies for E1-ATP class 1,2,3
/data/Movies/Dataset4
micrographs - multiframe
TIFF
TIFF
4333
54
1
54
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset4/*.tif
data/Particles/dataset4-all.star
data/Particles/Dataset4