REL
2019-09-19
2021-06-09
2021-06-09
200kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.65 A
0000-0002-8451-9947
Xueming
Li
School of Life Science, Tsinghua University
Tsinghua University, Room 240, New Biology Building
Beijing
Beijing
China
100084
0000-0002-8451-9947
Xueming
Li
School of Life Science, Tsinghua University
Tsinghua University, Room 240, New Biology Building
Beijing
Beijing
China
100084
0000-0003-3425-6672
Cong
Liu
Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
26 Qiuyue Road, Building #6, Rm 204
Shanghai
Shanghai
China
201210
Zhou H
Luo F
Luo Z
Li D
Liu C
Li X
9.5
10.6019/EMPIAR-10318
MicroED
EMD-0696
Zhou H
Luo F
Luo Z
Li D
Liu C
Li X
Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules
Analytical Chemistry
Anal. Chem.
United States
17
91
10996
11003
2019
English
10.1021/acs.analchem.9b01162
31334636
Electron diffraction image are saved in SMV/img format, with each img file covering an 1 degree wedge. The beam center information of each dataset is saved in the corresponding cec file and should be manually input to data processing softwares.
200kV MicroED data of FUS (37-42) SYSGYS
/data
diffraction images
SMV
SMV
8
1
SIGNED 16 BIT INTEGER
4096
51.0
4096
51.0
The data is collected on FEI Tecnai F20 200kV microscope. The electron wavelength is 0.02507 Angstrom. The final dataset is merged from 8 crystals. Each zip file contains one set of MicroED data (in SMV/img format) collected from one crystal. Each img file covers an 1 degree wedge (accumulating 0.0572 electron per squared Angstrom in a 5.72 seconds exposure). Since some data processing softwares (such as HKL2000) only support specific cameras used in X-ray crystallography, the pixel size of diffraction images has been set to 51 micrometers to imitate 4k x 4k ADSC Q210 camera, and the corresponding equivalent camera length has been saved in the header of img files and could be recognized by data processing softwares. The beam center information of each dataset is saved in the corresponding cec file and should be manually input to data processing softwares.