Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP.

REL 2019-09-27 2019-10-07 2019-10-07 Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP. 0000-0002-9727-6657 Moritz Treeck The Francis Crick Institute 1 Midland Road London United Kingdom NW1 1AT 0000-0002-9727-6657 Moritz Treeck The Francis Crick Institute 1 Midland Road London United Kingdom NW1 1AT Gary Ward Department of Microbiology and Molecular Genetics, Larner College of Medicine, University of Vermont 316 Stafford Hall, 95 Carrigan Drive Burlington VT United States 05405 Aoife Heaslip Department of Molecular & Cell Biology, University of Connecticut 91 Eagleville Road, Unit 3125 Storrs CT United States 06269-3125 Hunt A Russell MRG Wagener J Kent R Carmeille R Peddie CJ Collinson L Heaslip A Ward GE Treeck M 583.8 10.6019/EMPIAR-10327 FIB-SEM Hunt A Russell MRG Wagener J Kent R Carmeille R Peddie CJ Collinson L Heaslip A Ward GE Treeck M Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii eLife Elife 8 2019 English 10.7554/elife.50598 31577230

Image acquisition; 5 nm voxels: xy pixel set to 5 nm, FIB mill every 5 nm. SEM: 1.5 kV with 1 nA current, 10 μs dwell time. EsB detector grid voltage: 1200 V. Ion beam milling: 30 kV, 700 pA. Image processing; Zeiss Atlas 5.2.1.25; Gradient align Export of cropped region of interest exported as 8 bit TIF files Fiji; Invert Gaussian blur: Sigma 0.75 Unsharp mask: Radius (Sigma) 1 pixel, Mask weight 0.6 Rotate to straighten XY (D19: no rotation) Reslice: YZ assigned to XY plane Scale to 10 nm isotropic voxels IMOD; tif2mrc

Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP. /data/mrc micrographs - multiframe MRC MRC 1 1 SIGNED BYTE 1540 100.0 1254 100.0