REL
2019-11-26
2020-03-02
2020-03-02
Cryo-EM reconstruction of tau filaments extracted from the brains of three individuals with Corticobasal degeneration
0000-0002-0462-6540
Sjors
H.W.
Scheres
MRC laboratory of molecular biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-0462-6540
Sjors
H.W.
Scheres
MRC laboratory of molecular biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Zhang W
Tarutani A
Newell KL
Murzin AG
Matsubara T
Falcon B
Vidal R
Garringer HJ
Shi Y
Ikeuchi T
Murayama S
Ghetti B
Hasegawa M
Goedert M
Scheres SHW
2.8
10.6019/EMPIAR-10340
EMDB
EMD-10512
EMD-10514
Zhang W
Tarutani A
Newell KL
Murzin AG
Matsubara T
Falcon B
Vidal R
Garringer HJ
Shi Y
Ikeuchi T
Murayama S
Ghetti B
Hasegawa M
Goedert M
Scheres SHW
Novel tau filament fold in corticobasal degeneration
Nature
Nature
United Kingdom
2020
English
10.1038/s41586-020-2043-0
32050258
Unaligned movies for Case 1
/data/Movies/Case1
micrographs - multiframe
TIFF
TIFF
2567
40
1
40
32 BIT FLOAT
3838
1.15
3710
1.15
Movies/Case1 contains unaligned movies for Case1. The files were written as gain-normalised floating point MRC files by EPU and then converted into gain-unnormalised floating point TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.346 e/A2/frame. Please use Movies/Case1/gain-reference.mrc as a gain reference without any rotation or flipping.
Micrographs/Case1 contains aligned and summed micrographs.
Coordinates/Case1 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.
Particles/Case1 contains polished "shiny" particles used for final reconstructions.
data/Micrographs/Case1
data/Coordinates/Case1
data/Particles/Case1
Unaligned movies for Case 2
/data/Movies/Case2
micrographs - multiframe
TIFF
TIFF
4442
50
1
50
SIGNED BYTE
3838
1.15
3710
1.15
Movies/Case2 contains unaligned movies for Case2. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rates are 1.1 and 2.0 e/A2/frame for low-dose and high-dose datasets, respectively. Please use Movies/Case2/Movie-lowdose/gain.mrc and Movies/Case2/Movie-highdose/gain.mrc, respectively, as gain references without any rotation or flipping.
Micrographs/Case2 contains aligned and summed micrographs.
Coordinates/Case2 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.
Particles/Case2 contains polished "shiny" particles used for final reconstructions.
data/Micrographs/Case2
data/Coordinates/Case2
data/Particles/Case2
Unaligned movies for Case 3
/data/Movies/Case3
micrographs - multiframe
TIFF
TIFF
3114
48
1
48
SIGNED BYTE
3838
1.15
3710
1.15
Movies/Case3 contains unaligned movies for Case3. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.094 e/A2/frame. Please use Movies/Case3/gain.mrc as a gain reference without any rotation or flipping.
Micrographs/Case3 contains aligned and summed micrographs.
Coordinates/Case3 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.
Particles/Case3 contains polished "shiny" particles used for final reconstructions.
data/Micrographs/Case3
data/Coordinates/Case3
data/Particles/Case3