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        <keyDates>
            <depositionDate>2020-01-31</depositionDate>
            <releaseDate>2020-04-03</releaseDate>
            <updateDate>2020-02-04</updateDate>
        </keyDates>
        <title>Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-4813-5740</authorORCID>
            <firstName>Wataru</firstName>
            <lastName>Shihoya</lastName>
            <organization type="academic">Department of Biological Sciences, Graduate School of Science, The University of Tokyo</organization>
            <street>7-3-1 Hongo</street>
            <townOrCity>Bunkyoku</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-1813-7008</authorORCID>
            <firstName>Osamu</firstName>
            <lastName>Nureki</lastName>
            <organization type="academic">Department of Biological Sciences, Graduate School of Science, The University of Tokyo</organization>
            <street>7-3-1 Hongo</street>
            <townOrCity>Bunkyoku</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-3366-3586">Kobayashi K</author>
            <author authorORCID="0000-0003-4813-5740">Shihoya W</author>
            <author authorORCID="0000-0001-7463-8398">Nishizawa T</author>
            <author>Kadji FMN</author>
            <author authorORCID="0000-0001-9435-1896">Aoki J</author>
            <author>Inoue A</author>
            <author authorORCID="0000-0003-1813-7008">Nureki O</author>
        </authorsList>
        <datasetSize units="TB">3.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-10351</entryDOI>
        <experimentType>EMDB</experimentType>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-0940</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-3366-3586" order="1">Kobayashi K</author>
                    <author authorORCID="0000-0003-4813-5740" order="2">Shihoya W</author>
                    <author authorORCID="0000-0001-7463-8398" order="3">Nishizawa T</author>
                    <author order="4">Kadji FMN</author>
                    <author authorORCID="0000-0001-9435-1896" order="5">Aoki J</author>
                    <author order="6">Inoue A</author>
                    <author authorORCID="0000-0003-1813-7008" order="7">Nureki O</author>
                    <title>Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat. Struct. Mol. Biol.</journalAbbreviation>
                    <country>Japan</country>
                    <issue>3</issue>
                    <volume>27</volume>
                    <firstPage>274</firstPage>
                    <lastPage>280</lastPage>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-020-0386-8</externalReferences>
                    <externalReferences type="pubmed">32157248</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned movies for the human PAC1 receptor coupled to Gs (dimer)</name>
        <directory>/data/Movies/Dataset</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>2895</numImagesOrTiltSeries>
        <framesPerImage>64</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>64</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.8346</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.8346</pixelHeight>
        </dimensions>
        <details>Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.</details>
        <segmentationList/>
        <micrographsFilePattern>data/Movies/Dataset/*.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern>data/Particles/shiny.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/Particles/Dataset</pickedParticlesDirectory>
    </imageSet>
</entry>
