REL
2020-02-07
2020-11-18
2020-12-09
CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 2
0000-0003-1579-7561
Yang
Shi
MRC-LMB
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-5214-7886
Michel
Goedert
MRC-LMB
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-0462-6540
Sjors
H.W.
Scheres
MRC-LMB
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Schweighauser M
Shi Y
Tarutani A
Kametani F
Murzin AG
Ghetti B
Matsubara T
Tomita T
Ando T
Hasegawa K
Murayama S
Yoshida M
Hasegawa M
Scheres SHW
Goedert M
1.1
10.6019/EMPIAR-10358
EMDB
EMD-10651
EMD-10652
Schweighauser M
Shi Y
Tarutani A
Kametani F
Murzin AG
Ghetti B
Matsubara T
Tomita T
Ando T
Hasegawa K
Murayama S
Yoshida M
Hasegawa M
Scheres SHW
Goedert M
Structures of α-synuclein filaments from multiple system atrophy
Nature
Nature
United Kingdom
7825
585
464
469
2020
English
10.1038/s41586-020-2317-6
32461689
Unaligned multi-frame movies
/data/Movies
micrographs - multiframe
TIFF
TIFF
5172
48
1
48
UNSIGNED BYTE
3838
1.15
3710
1.15
Movies were recorded automatically using EPU on a K2 camera in counting mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 10.8 second movies contain 48 frames and the pixel size is 1.15 Å/px. The total dose per movie is ~47.5 e/Å^2. The gain reference image is included.
Dose-weighted aligned micrographs
/data/Micrographs
micrographs - single frame
MRC
MRC
5172
1
32 BIT FLOAT
3838
1.15
3710
1.15
Movie frames were corrected for beam-induced motion and dose-weighted using RELION’s motion-correction implementation with following arguments:
--first_frame_sum 1 --last_frame_sum -1 --use_own --j 5 --bin_factor 1 --bfactor 150 --angpix 1.15 --voltage 300 --dose_per_frame 0.81 --preexposure 0 --patch_x 4 --patch_y 4 --gainref gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting
Polished particle stacks
/data/Particles
picked particles - single frame - processed
MRCS
MRCS
3030
1
32 BIT FLOAT
220
1.15
220
1.15
Bayesian polishing was performed in RELION-3.0. STAR files of both Type2-1 and Type2-2 with the metadata of the final refined segments are included.