<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10383" schemaVersion="0.65" public="true">
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        <keyDates>
            <depositionDate>2020-03-05</depositionDate>
            <releaseDate>2021-11-09</releaseDate>
            <updateDate>2023-05-02</updateDate>
        </keyDates>
        <title>CryoEM structure of human LRRC8D</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-1813-7008</authorORCID>
            <firstName>Osamu</firstName>
            <lastName>Nureki</lastName>
            <organization type="academic">Department of Biological Sciences, School of Science, The University of Tokyo</organization>
            <street>7-3-1 Hongo</street>
            <townOrCity>Bunkyo-ku</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-1813-7008</authorORCID>
            <firstName>Osamu</firstName>
            <lastName>Nureki</lastName>
            <organization type="academic">Department of Biological Sciences, School of Science, The University of Tokyo</organization>
            <street>7-3-1 Hongo</street>
            <townOrCity>Bunkyo-ku</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Nakamura R</author>
            <author authorORCID="0000-0003-1756-5764">Kasuya G</author>
            <author>Yokoyama T</author>
            <author authorORCID="0000-0002-7997-2149">Shirouzu M</author>
            <author authorORCID="0000-0002-4136-5685">Ishitani R</author>
            <author authorORCID="0000-0003-1813-7008">Nureki O</author>
        </authorsList>
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        <entryDOI>10.6019/EMPIAR-10383</entryDOI>
        <experimentType>EMDB</experimentType>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-30029</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Nakamura R</author>
                    <author authorORCID="0000-0002-5719-4884" order="2">Numata T</author>
                    <author authorORCID="0000-0003-1756-5764" order="3">Kasuya G</author>
                    <author order="4">Yokoyama T</author>
                    <author authorORCID="0000-0001-7463-8398" order="5">Nishizawa T</author>
                    <author authorORCID="0000-0002-6186-6647" order="6">Kusakizako T</author>
                    <author order="7">Kato T</author>
                    <author order="8">Hagino T</author>
                    <author order="9">Dohmae N</author>
                    <author order="10">Inoue M</author>
                    <author authorORCID="0000-0002-0348-1634" order="11">Watanabe K</author>
                    <author authorORCID="0000-0002-5005-6438" order="12">Ichijo H</author>
                    <author authorORCID="0000-0001-7656-8194" order="13">Kikkawa M</author>
                    <author authorORCID="0000-0002-7997-2149" order="14">Shirouzu M</author>
                    <author authorORCID="0000-0002-3509-2553" order="15">Jentsch TJ</author>
                    <author order="16">Ishitani R</author>
                    <author order="17">Okada Y</author>
                    <author authorORCID="0000-0003-1813-7008" order="18">Nureki O</author>
                    <title>Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation</title>
                    <journal>Communications biology</journal>
                    <journalAbbreviation>Commun Biol</journalAbbreviation>
                    <country>Japan</country>
                    <issue>1</issue>
                    <volume>3</volume>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s42003-020-0951-z</externalReferences>
                    <externalReferences type="pubmed">32415200</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned movies of human LRRC8D isoform</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3399</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>7420</imageWidth>
            <pixelWidth>1.49</pixelWidth>
            <imageHeight>7676</imageHeight>
            <pixelHeight>1.49</pixelHeight>
        </dimensions>
        <details>Movies were collected on Talos Arctica at 0.745 A / super-resolution pixel (that is, 1.49 A / physical pixel). Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2". Seven datasets were combined to yield the structure.
Dose rate (shown on SerialEM) of Datasets 0 and 1 are 1.245 e/A2/frame</details>
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        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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