REL
2020-03-26
2021-08-13
2021-08-13
Human apo ferritin frozen on TEM grid with amorphous carbon supporting film
0000-0002-0351-5144
Fei
Sun
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road
Beijing
Beijing
China
100101
0000-0002-0351-5144
Fei
Sun
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road
Beijing
Beijing
China
100101
Huang X
Zhang L
Wen Z
Chen H
Li S
Ji G
Yin CC
Sun F
226.5
10.6019/EMPIAR-10405
EMDB
EMD-30083
Huang X
Zhang L
Wen Z
Chen H
Li S
Ji G
Yin CC
Sun F
Amorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation
Progress in biophysics and molecular biology
Prog Biophys Mol Biol
156
3
13
2020
English
10.1016/j.pbiomolbio.2020.07.009
32758492
The micrographs are collected on FEI Titan Krios transmission electron microscope (ThermoFisher Scientific) at 300 kV with Gatan K2 Summit camera. The pixel size was 0.41 Å in the super resolution mode, corresponding to the physical pixel size of 0.82 Å. After the pixel size refinement during model building, the corrected physical pixel size is 0.87 Å. Each image was exposed for 5.2 sec in a dose rate of 11.5 e/Å2/sec, resulting in a total dose of ~ 60 e/ Å2.
Human apo ferritin frozen on TEM grid with amorphous carbon supporting film
/data/C/micrographs
micrographs - multiframe
TIFF
TIFF
743
32
UNSIGNED BYTE
7676
0.435
7420
0.435
The raw data were taken in K2 super resolution mode. There are 743 multi-frame stacks without gain correction, motion correction and distortion correction in TIFF format with LZW compression of 8bit. The gain file were also provided in data/C/gain.mrc (7676*7420, 32bit). The distortion parameter were distotaion angle of 26.1, major scale of 1.019 and minor scale of 1. The total dose was 60 e/A2.
Particle positions in corresponding coordinate start file were used to extract particles from bin2 micrographs for final reconstruction.
data/C/micrographs/C2_*.tif
data/C/particles.star
data/C/extracted_particles