REL
2020-05-11
2020-06-30
2022-02-08
Soft X-ray Tomography of mock-infected U2OS cells
0000-0001-8091-9057
Maria
Harkiolaki
Diamond Light Source
Harwell Science & Innovation Campus Didcot
Didcot
Oxfordshire
United Kingdom
OX11 0DE
0000-0001-8091-9057
Maria
Harkiolaki
Diamond Light Source
Harwell Science & Innovation Campus Didcot
Didcot
Oxfordshire
United Kingdom
OX11 0DE
Kounatidis I
Stanifer ML
Phillips MA
Paul-Gilloteaux P
Heiligenstein X
Wang H
Okolo CA
Fish TM
Spink MC
Stuart DI
Davis I
Boulant S
Grimes JM
Dobbie IM
Harkiolaki M
2.3
10.6019/EMPIAR-10413
CLXM
Kounatidis I
Stanifer ML
Phillips MA
Paul-Gilloteaux P
Heiligenstein X
Wang H
Okolo CA
Fish TM
Spink MC
Stuart DI
Davis I
Boulant S
Grimes JM
Dobbie IM
Harkiolaki M
3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway
Cell
Cell
2
182
515
530.e17
2020
English
10.1016/j.cell.2020.05.051
32610083
S-BIAD17
S-BIAD18
S-BIAD19
S-BIAD20
Tomogram of reovirus mock-infected U2OS cells (Area 2)
/data
tilt series
MRC
MRC
1
1
32 BIT FLOAT
946
160.0
946
160.0
Tomogram of reovirus mock-infected U2OS cells (Area2)
EMPIAR entry 10413: U2OS_Reo_MockInfected_Area2_Reconstruction (S-BIAD17)
Note:
Associated with EMPIAR entry 10412 (adjacent/overlapping FOVs)
Sample characteristics:
Substrate = Quantifoil F1 2/2
Fiducials = Au 250nm nanospheres
Data collection:
Data collected at beamline B24 at Diamond Light source
Data collected 18th Feb 2019
Allocation award ID MX21046
Tilt series:
-65deg to 65deg
0.5deg step
Total frames 261
0.5sec exposure
Microscope make:
Zeiss UltraXRM-S220C
Data collection software:
XRM Controller
Microscope setup:
Energy: 500eV
Magnification = x812.5
FOV = 16microns
pixel size = 16x16nm
Zoneplate = 40nm
Data processing:
Type = Automated
Software = Batchruntomo
Alignment protocol = SIRT