REL
2020-06-10
2020-07-30
2020-07-30
Cryo-EM structures of calcium homeostasis modulator (CALHM) channels
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyo-ku
Tokyo
Japan
113-0033
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo
Bunkyo-ku
Tokyo
Japan
113-0033
Demura K
Kusakizako T
Shihoya W
Hiraizumi M
Shimada H
Yamashita K
Nishizawa T
Nureki O
6.8
10.6019/EMPIAR-10444
EMDB
EMD-0919
EMD-0920
EMD-0921
EMD-0922
EMD-0923
Demura K
Kusakizako T
Shihoya W
Hiraizumi M
Nomura K
Shimada H
Yamashita K
Nishizawa T
Taruno A
Nureki O
Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies
Science Advances
Sci Adv
29
6
2020
English
10.1126/sciadv.aba8105
Unaligned movies for OlCALHM1
/data/Movies/Dataset0
micrographs - multiframe
TIFF
TIFF
3075
54
1
54
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset0/*.tif
data/Particles/dataset0-all.star
data/Particles/Dataset0
Unaligned movies for HsCALHM2
/data/Movies/Dataset1
micrographs - multiframe
TIFF
TIFF
2110
60
1
60
SIGNED 16 BIT INTEGER
4096
0.8346
4096
0.8346
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.
data/Movies/Dataset1/*.tiff
data/Particles/dataset1-all.star
data/Particles/Dataset1
Unaligned movies for CeCLHM-1
/data/Movies/Dataset2
micrographs - multiframe
TIFF
TIFF
2906
48
1
48
SIGNED 16 BIT INTEGER
4096
0.8346
4096
0.8346
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.
data/Movies/Dataset2/*.tif
data/Particles/dataset2-all.star
data/Particles/Dataset2
Unaligned movies for OlCALHM1-HsCALHM2 chimera
/data/Movies/Dataset3
micrographs - multiframe
TIFF
TIFF
2808
48
1
48
UNSIGNED BYTE
5760
0.83
4092
0.83
Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).
data/Movies/Dataset3/*.tif
data/Particles/dataset3-[12].star
data/Particles/Dataset3_[12]