REL 2020-09-01 2020-11-11 2020-11-11 0000-0002-7478-7390 Makoto Miyata Graduate School of Science, Osaka City University Osaka Japan 558-8585 0000-0003-2911-5875 Keiichi Namba Graduate School of Frontier Biosciences, Osaka University Osaka Japan 565-0871 Vizarraga D Kawamoto A Matsumoto U Illanes R Pérez-Luque R Martín J Mazzolini R Bierge P Pich OQ Espasa M Sanfeliu I Esperalba J Fernández-Huerta M Scheffer MP Pinyol J Frangakis AS Lluch-Senar M Mori S Shibayama K Kenri T Kato T Namba K Fita I Miyata M Aparicio D 8.0 10.6019/EMPIAR-10518 EMDB EMD-30233 Vizarraga D Kawamoto A Matsumoto U Illanes R Pérez-Luque R Martín J Mazzolini R Bierge P Pich OQ Espasa M Sanfeliu I Esperalba J Fernández-Huerta M Scheffer MP Pinyol J Frangakis AS Lluch-Senar M Mori S Shibayama K Kenri T Kato T Namba K Fita I Miyata M Aparicio D Nature communications Nat Commun 1 11 2020 English 10.1038/s41467-020-18777-y 33057023 Unaligned 136-frame movies of human pathogen Mycoplasma pneumoniae P1 /data/P1-not-on-GO micrographs - multiframe TIFF TIFF 512 136 SIGNED 16 BIT INTEGER 4096 0.88 4096 0.88

human pathogen Mycoplasma pneumoniae P1(particles have preferred orientations). Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector. A total of 512 movies were obtained in the electron counting mode with a physical pixel size of 0.88 Å/pixel and total dose of 60 e/Å2 with 63.2 s exposure time. The data were automatically collected using EPU software with a defocus range of -1.25 to -2.75 µm and were fractionated into 136 movie frames.

Unaligned 78-frame movies of human pathogen Mycoplasma pneumoniae P1 /data/P1-on-GO micrographs - multiframe TIFF TIFF 5469 78 SIGNED 16 BIT INTEGER 4096 0.69 4096 0.69

human pathogen Mycoplasma pneumoniae P1 on graphene oxide grid. Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector. A total of 5469 movies were obtained in the electron counting mode with a physical pixel size of 0.69 Å/pixel and total dose of 60 e/Å2 with 36.33 s exposure time. The data were automatically collected using EPU software with a defocus range of -0.75 to -2.75 µm and were fractionated into 78 movie frames.