REL 2020-11-09 2020-11-20 2020-11-20 0000-0003-0994-2937 Ohad Medalia Department of Biochemistry, University of Zurich Winterthurerstr. 190 Zurich Zurich Switzerland 8057 0000-0003-0994-2937 Ohad Medalia Department of Biochemistry, University of Zurich Winterthurerstr. 190 Zurich Zurich Switzerland 8057 Ohad Medalia OM 4.0 10.6019/EMPIAR-10548 EMDB EMD-10737 Rajaa Boujemaa-Paterski RBP Bruno Martins BM Matthias Eibauer ME Charlie Beales CB Benjamin Geiger BG Medalia O eLife Elife United Kingdom 9 2020 English 10.7554/elife.53990 33185186

The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).

Tomograms of actin bundles induced by full length vinculin /data tilt series MRC MRC 2 61 32 BIT FLOAT 1024 1.7 1024 1.7

The tilt-series were acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 42,000 x with 0.17 nm pixel size, and recorded with a 2° increment over a -60° to 60° angular range (accumulating ~70 electrons per squared Angstrom) at a -4 defocus. The tilt-series were then down-sampled, motion-corrected, contrast transfer function corrected and reconstructed in volumes with a size of 1024x1024x512 voxel (with 8x8 total down-sampling and a voxel size of 13.6 Angstrom).