REL 2020-11-17 2020-11-24 2021-06-30 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 Danev R 659.0 10.6019/EMPIAR-10559 EMDB EMD-30683 Danev R Yanagisawa H Kikkawa M Microscopy (Oxford, England) Microscopy (Oxf) 1 1 1 2 2021 English 10.1093/jmicro/dfab016 33969878 Unaligned multi-frame non-gain-normalized movies in LZW compressed TIFF format /data micrographs - multiframe TIFF TIFF 2391 76 UNSIGNED BYTE 11520 0.1997 8184 0.1997

Please see the "dataset_details_apoF.txt" file for details. To test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020. This was the first dataset in the series. Sample: mouse heavy chain apoferritin Grid: UltrAuFoil 300 mesh R1.2/1.3 Cemera: Gatan K3 post a BioQuantum energy filter Camera mode: super resolution, CDS TEM mode: EFTEM NanoProbe Mag: x215k C2 aperture: 50 um Spot: 4 Beam diameter: 1.55 um EF slit: 15 eV Data collection software: SerialEM Acquisition method: beam-image shift with beam-tilt compensation Acquisition pattern: 3 x 3 holes, 1 image in the center of each hole Physical Pixel: 0.3994 A/pix SuperRes pixel: 0.1997 A/pix Exposure rate: 3.42 e/pix/s (CDS) Exposure time: 3.04 s Total exposure: 64.5 e/A2 Frames: 76 Defocus: 0.2 - 0.8 um Movies: 2391 Data format: non-gain-normalized LZW compressed TIFFs Gain reference: "apoF_gain.mrc" prepared from the data Final particles: 181k Particles file: "run_data.star" Optics groups: per-acquisition position and per hour Resolution (with Ewald correction): 1.31 A Rosenthal-Henderson B-factor: 38.7 A^2 Rado November 19, 2020

data/*.tif data/run_data.star