REL 2020-11-19 2020-12-11 2023-02-20 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 Danev R 879.2 10.6019/EMPIAR-10568 EMDB EMD-30684 Danev R Yanagisawa H Kikkawa M Microscopy (Oxford, England) Microscopy (Oxf) 1 1 1 2 2021 English 10.1093/jmicro/dfab016 33969878 Unaligned multi-frame non-gain-normalized EER movies in TIFF format /data/Images-Disc1 micrographs - multiframe TIFF EER 3540 720 BIT 4096 0.3948 4096 0.3948

Please check the "dataset_details_apoF_EPU.txt" for details. To test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020. This was the third dataset in the series. Sample: mouse heavy chain apoferritin Grid: UltrAuFoil 300 mesh R1.2/1.3 Cemera: Falcon 4 installed at pre-fitler camera position Camera mode: EER TEM mode: NanoProbe Mag: x195k C2 aperture: 50 um Spot: 6 Beam diameter: 0.65 um Data collection software: EPU Acquisition method: beam-image shift without beam-tilt compensation Acquisition pattern: 1 x 1 holes, 4 images around the periphery of the hole Physical Pixel: 0.3948 A/pix EER 2x oversamples pixel: 0.1974 A/pix Exposure rate: 2.74 e/pix/s (measured in EPU) Exposure time: 3.0 s Total exposure: 52.7 e/A2 Frames: 720 EER frames, processed with 10 frames/fraction resulting in 72 fractions Defocus: 0.2 - 1.0 um Movies: 3540 Data format: non-gain-normalized EER encapsulated in TIFF format Gain reference: "gain_2xUps_8k.mrc" prepared with the command "relion_estimate_gain --i Import/job001/movies.star --o gain_2xUps_8k.mrc --eer_upsampling 2 --dont_invert --j 30" from the data Final particles: 135k Particles file: "run_data.star" Optics groups: per-acquisition position and per hour Resolution (with Ewald correction): 1.43 A Rosenthal-Henderson B-factor: 42.9 A^2 Rado November 24, 2020