REL 2020-11-25 2020-12-07 2021-07-01 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 0000-0001-6406-8993 Radostin Danev The University of Tokyo 7-3-1 Hongo, Bunkyo-ku Toyko Japan 113-0033 Danev R 1.2 10.6019/EMPIAR-10579 EMDB EMD-30686 Danev R Yanagisawa H Kikkawa M Microscopy (Oxford, England) Microscopy (Oxf) 1 1 1 2 2021 English 10.1093/jmicro/dfab016 33969878 Unaligned multi-frame non-gain-normalized movies in LZW compressed TIFF format /data/0706.apoF.1_set3_noEF micrographs - multiframe TIFF TIFF 4184 76 UNSIGNED BYTE 11520 0.1997 8184 0.1997

Please see the "dataset_details_apoF_set3_noEF.txt" file for details. To test the overall performance of the Krios G4 at The University of Tokyo, five datasets were collected from the same apoferritin grid in the beginning of July 2020. This was the last, fifth dataset in the series. Sample: mouse heavy chain apoferritin Grid: UltrAuFoil 300 mesh R1.2/1.3 Cemera: Gatan K3 post a BioQuantum energy filter Camera mode: super resolution, CDS TEM mode: EFTEM NanoProbe Mag: x215k C2 aperture: 50 um Spot: 6 Beam diameter: 0.65 um EF slit: out Data collection software: SerialEM Acquisition method: beam-image shift with beam-tilt compensation Acquisition pattern: 1 x 1 holes, 4 images around the periphery of the hole Physical Pixel: 0.3994 A/pix SuperRes pixel: 0.1997 A/pix Exposure rate: 3.62 e/pix/s (CDS) Exposure time: 3.04 s Total exposure: 69.0 e/A2 Frames: 76 Defocus: 0.2 - 0.9 um Movies: 4184 Data format: non-gain-normalized LZW compressed TIFFs Gain reference: "apoF_set3_noEF_gain.mrc" prepared from the data Final particles: 113k Particles file: "run_data.star" Optics groups: per-acquisition position Resolution (with Ewald correction): 1.43 A Rosenthal-Henderson B-factor: 42.7 A^2 Note: The air-conditioning temperature of the Krios G4 room was reduced by 2 degC around noon on the second day (July 13th) which caused a gradual increase in beam tilt/coma in the data. Therefore, only data from the first two acquired grid squares (314 and 321) was used. Rado November 26, 2020

data/0706.apoF.1_set3_noEF/*.tif data/run_data.star