REL
2020-11-10
2021-11-26
2021-11-26
Cryo-EM reconstruction of heavy chain mouse apoferritin, 8 datasets testing dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. [1143 multi-frame micrographs composed of 120 frames in LZW compressed TIFF format
0000-0002-4413-2502
Ming
Sun
UCSF
San Francisco
CA
United States
94158
0000-0003-3512-695X
David
Agard
UCSF
San Francisco
CA
United States
94158
Sun M
Azumaya CM
Tse E
Bulkley DP
Harrington MB
Gilbert G
Frost A
Southworth D
Verba KA
Cheng Y
Agard DA
8.5
10.6019/EMPIAR-10588
EMDB
EMD-22972
Sun M
Azumaya CM
Tse E
Bulkley DP
Harrington MB
Gilbert G
Frost A
Southworth D
Verba KA
Cheng Y
Agard DA
Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM
Journal of structural biology
J Struct Biol
3
213
2021
English
10.1016/j.jsb.2021.107745
33984504
Datasets used for cryo-EM reconstruction of heavy chain mouse apoferritin, resulting from optimization of dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. A total of 8 datasets. Contains 1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference TIFF for the 8 e-/pix/s dose rate dataset at 105,000x nominal magnification collected in CDS mode. Also at 105kx, we include 1152 CDS movies collected at 16 e-/pix/s, 1143 CDS movies collected at 4 e-/pix/s as well as 1143 movies for 8 e-/pix/s collected in standard mode. At 64kx we include 1152 CDS movies collected at 16 e-/pix/s, 1080 CDS movies collected at 8 e-/pix/s, as well as 1143 movies for 16 e-/pix/s and 1107 movies for 8 e-/pix/s in standard mode.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3
/data/apof-105kx-8eps-CDS
micrographs - multiframe
TIFF
TIFF
1143
120
UNSIGNED BYTE
11520
0.417
8184
0.417
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3
/data/apof-105kx-8eps_nonCDS
micrographs - multiframe
TIFF
TIFF
1143
120
UNSIGNED BYTE
11520
0.417
8184
0.417
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in standard mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3
/data/apof-105kx-4eps-CDS
micrographs - multiframe
TIFF
TIFF
1143
120
UNSIGNED BYTE
11520
0.417
8184
0.417
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 4 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3
/data/apof-105kx-16eps-CDS
micrographs - multiframe
TIFF
TIFF
1152
120
UNSIGNED BYTE
11520
0.417
8184
0.417
1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3 at lower magnification
/data/apof-64kx-8eps-CDS
micrographs - multiframe
TIFF
TIFF
1080
120
UNSIGNED BYTE
11520
0.673
8184
0.673
1080 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3 at lower magnification
/data/apof-64kx-8eps-nonCDS
micrographs - multiframe
TIFF
TIFF
1107
120
UNSIGNED BYTE
11520
0.673
8184
0.673
1107 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in CDS mode from Gatan K3 at lower magnification
/data/apof-64kx-16eps-CDS
micrographs - multiframe
TIFF
TIFF
1152
120
UNSIGNED BYTE
11520
0.673
8184
0.673
1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.
apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in standard mode from Gatan K3 at lower magnification
/data/apof-64kx-16eps-nonCDS
micrographs - multiframe
TIFF
TIFF
1143
120
UNSIGNED BYTE
11520
0.673
8184
0.673
1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.