Nanoscale view of the Clostridium thermocellum cellulosome during cellulose degradation reveals an ecological strategy leading to phenotypic heterogeneity

REL 2020-12-14 2021-01-08 2022-02-08 Nanoscale view of the Clostridium thermocellum cellulosome during cellulose degradation reveals an ecological strategy leading to phenotypic heterogeneity 0000-0001-6636-8818 Itzhak Mizrahi Faculty of Natural Sciences, Ben-Gurion University of the Negev Beer-Sheva Israel 8499000 0000-0003-0994-2937 Ohad Medalia Department of Biochemistry, University of Zurich Zurich Switzerland 8052 Tatli M Moraïs S Tovar-Herrera OE Bomble Y Bayer EA Medalia O Mizrahi I 1004.3 10.6019/EMPIAR-10593 FIB-SEM Tatli M Moraïs S Tovar-Herrera OE Bomble Y Bayer EA Medalia O Mizrahi I Nanoscale view of the Clostridium thermocellum cellulosome during cellulose degradation reveals an ecological strategy leading to phenotypic heterogeneity Aligned tilt series of C.thermocellum grown in MCC for 15 hr /data tilt series MRC MRC 37 6 SIGNED 16 BIT INTEGER 3708 1.35 3838 1.35

Includes a tilt-series that was acquired at a dose-fractionated frame rate of 1 frame per 0.2 seconds, at a magnification of 105,000 x with 0.135 nm pixel size, and recorded with a 3° increment over a -48° to 60° angular range (accumulating ~140 e-/Å2) at a -4 defocus.