A lamin A/C variant causing striated muscle disease provides insights into filament organization

REL 2020-12-16 2021-01-08 2021-06-18 A lamin A/C variant causing striated muscle disease provides insights into filament organization 0000-0003-0994-2937 Ohad Medalia Department of Biochemistry, University of Zurich Zurich Switzerland 8052 0000-0003-0994-2937 Ohad Medalia Department of Biochemistry, University of Zurich Zurich Switzerland 8052 Tatli M 2.7 10.6019/EMPIAR-10601 EMDB EMD-13056 EMD-13061 Kronenberg-Tenga R Tatli M Eibauer M Wu W Shin JY Bonne G Worman HJ Medalia O A lamin A/C variant causing striated muscle disease provides insights into filament organization Journal of cell science J Cell Sci Switzerland 6 134 2021 English 10.1242/jcs.256156 33536248 Tilt-series of LmnaH222P/H222P revealing lamin meshwork in nuclease treated and FIB-milled nucleus /data tilt series MRC MRC 2 41 SIGNED 16 BIT INTEGER 3708 2.2 3838 2.2

Both nuclease treated near native and FIB-milled LmnaH222P/H222P tilt-series were acquired at a dose-fractionated of 1 frame per 0.2 seconds, at a magnification of 64 000 with a 0.22 nm pixel size and an angular range of -60° to 60°. Nuclease treated tilt series were acquired with bidirectional scheme and recorded with a 2° increment (accumulating ~140 e-/Å2) at a -6 defocus and aligned in imod. FIB-milled raw tilt series were acquired with dose-symmetric scheme and recorded with a 3° increment (accumulating ~155 e-/Å2) at a -4 defocus.