REL
2021-02-11
2021-03-05
2021-03-05
Cryo-EM of Multiple System Atrophy seeded assembly of alpha-synuclein filaments
0000-0002-2152-1476
Sofia
Karin Anna
Lövestam
MRC LMB Cambridge
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-0462-6540
Sjors
Scheres
MRC LMB Cambridge
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Lövestam S
Goedert M
Scheres S
1.1
10.6019/EMPIAR-10640
EMDB
EMD-12264
EMD-12265
EMD-12266
EMD-12267
EMD-12268
EMD-12269
Lövestam S
Schweighauser M
Matsubara T
Murayama S
Tomita T
Ando T
Hasegawa K
Yoshida M
Tarutani A
Hasegawa M
Goedert M
Scheres S
Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy
FEBS Open Bio
FEBS Open Bio
United Kingdom
2021
English
10.1002/2211-5463.13110
33548114
Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 1
/data/EMPIAR_upload/Movies/Case1
micrographs - multiframe
TIFF
TIFF
2175
40
1
40
UNSIGNED 16 BIT INTEGER
3838
1.1
3710
1.1
Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios II). The exposure was at 36.7 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case1-gain-ref.MRC. Have fun.
data/EMPIAR_upload/Movies/Case1/*.tif
data/EMPIAR_upload/ManualPick/Case1/job004/Tiff/*.star
data/EMPIAR_upload/ManualPick/Case1/job004
Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2
/data/EMPIAR_upload/Movies/Case2
micrographs - multiframe
TIFF
TIFF
1294
40
1
40
UNSIGNED 16 BIT INTEGER
3838
1.14
3710
1.14
Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios I). The exposure was at 32.6 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case2-gain-ref.MRC. Have fun twice.
data/EMPIAR_upload/Movies/Case2/*.tif
data/EMPIAR_upload/ManualPick/Case2/job004/Tiff/*.star
data/EMPIAR_upload/ManualPick/Case2/job004
Unaligned movies of alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 5
/data/EMPIAR_upload/Movies/Case5
micrographs - multiframe
TIFF
TIFF
1265
40
1
40
UNSIGNED 16 BIT INTEGER
3838
1.14
3710
1.14
Unaligned and non-gain corrected, 40-frame movies (K2 counting mode) were collected with EPU using a Titan Krios operating at 300 kV (LMB Krios I). The exposure was at 37.5 e/A^2) with a defocus range of -2.8 to -1.5 um. Micrographs were manually picked in relion using start-end helical picking. Gain reference is in Movies/Case5-gain-ref.MRC. Have fun thrice.
data/EMPIAR_upload/Movies/Case5/*.tif
data/EMPIAR_upload/ManualPick/Case5/job005/Micrographs/*.star
data/EMPIAR_upload/ManualPick/Case5/job005
Type 1A particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type1A
picked particles - single frame - processed
MRCS
MRCS
67619
40
32 BIT FLOAT
256
1.14
256
1.14
Polished particles for Type1A
Type 2A particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type2A
picked particles - single frame - processed
MRCS
MRCS
52502
40
32 BIT FLOAT
256
1.14
256
1.14
Polished particles for Type 2A
Type 1B particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type1B
picked particles - single frame - processed
MRCS
MRCS
33479
40
32 BIT FLOAT
256
1.1
256
1.1
Polished particles for Type 1B
Type 2B particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type2B
picked particles - single frame - processed
MRCS
MRCS
87092
40
32 BIT FLOAT
256
1.1
256
1.1
Polished particles for Type 2B
Type 2AB particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type2AB
picked particles - single frame - processed
MRCS
MRCS
57358
40
32 BIT FLOAT
256
1.1
256
1.1
Polished particles for Type 2AB
Type 3 particles after Bayesian polishing
/data/EMPIAR_upload/PolishedParticles/Type3
picked particles - single frame - processed
MRCS
MRCS
71131
40
32 BIT FLOAT
256
1.14
256
1.14
Polished particles for Type 3