<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10740" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-06-07</depositionDate>
            <releaseDate>2022-02-25</releaseDate>
            <updateDate>2022-02-25</updateDate>
        </keyDates>
        <title>Cryo-EM of Mechanosensitive Ion Channel Flycatcher1 in GDN</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-4392-3898</authorORCID>
            <firstName>Sebastian</firstName>
            <lastName>Jojoa-Cruz</lastName>
            <organization type="academic">The Scripps Research Institute</organization>
            <street>10550 North Torrey Pines Road</street>
            <townOrCity>San Diego</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>92037</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-7153-3769</authorORCID>
            <firstName>Andrew</firstName>
            <middleName>Barrett</middleName>
            <lastName>Ward</lastName>
            <organization type="academic">The Scripps Research Institute</organization>
            <street>10550 North Torrey Pines Road</street>
            <townOrCity>San Diego</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>92037</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-4392-3898">Jojoa-Cruz S</author>
            <author authorORCID="0000-0002-4135-5356">Saotome K</author>
            <author authorORCID="0000-0001-9445-6671">Lee WH</author>
            <author authorORCID="0000-0003-0726-7034">Patapoutian A</author>
            <author authorORCID="0000-0001-7153-3769">Ward AB</author>
        </authorsList>
        <datasetSize units="TB">5.7</datasetSize>
        <entryDOI>10.6019/EMPIAR-10740</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-24186</emdbEntry>
            <emdbEntry>EMD-24187</emdbEntry>
            <emdbEntry>EMD-24188</emdbEntry>
            <emdbEntry>EMD-24189</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-4392-3898" order="1">Jojoa-Cruz S</author>
                    <author authorORCID="0000-0002-4135-5356" order="2">Saotome K</author>
                    <author authorORCID="0000-0003-4886-9824" order="3">Tsui CCA</author>
                    <author authorORCID="0000-0001-9445-6671" order="4">Lee WH</author>
                    <author authorORCID="0000-0001-6360-7959" order="5">Sansom MSP</author>
                    <author authorORCID="0000-0001-9580-3380" order="6">Murthy SE</author>
                    <author authorORCID="0000-0003-0726-7034" order="7">Patapoutian A</author>
                    <author authorORCID="0000-0001-7153-3769" order="8">Ward AB</author>
                    <title>Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>13</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-022-28511-5</externalReferences>
                    <externalReferences type="pubmed">35165281</externalReferences>
                    <details>Raw movies and gain references for two datasets. More information about the datasets are in the file "Description_FLYC1datasets". Final particle stack used for the C1 map is also included.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of FLYC1 in GDN, dataset 1</name>
        <directory>/data/EMPIAR_sjojoa/frames/19may24k/rawdata</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2648</numImagesOrTiltSeries>
        <framesPerImage>43</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>particles.star file corresponds to only particles from this dataset (dataset 1) and not dataset 2. Only particles from dataset 1 were used for bayesian polishing and final refinements to obtain the final C1 volume (before symmetry expansion).
Description_FLYC1datasets_v2.txt file contains further information about data collection.</details>
        <segmentationList/>
        <micrographsFilePattern>data/EMPIAR_sjojoa/frames/19may24k/rawdata/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/EMPIAR_sjojoa/particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/EMPIAR_sjojoa</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of FLYC1 in GDN, dataset 2</name>
        <directory>/data/EMPIAR_sjojoa/frames/19jul28d/rawdata</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2648</numImagesOrTiltSeries>
        <framesPerImage>42</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/EMPIAR_sjojoa/frames/19jul28d/rawdata/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Gain references for dataset 1 and dataset 2</name>
        <directory>/data/EMPIAR_sjojoa/gains</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>2</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Gain references for each dataset:
-Gain reference for dataset 1: 19may24k_ref.mrc
-Gain reference for dataset 2: 19jul28d_ref.mrc</details>
        <segmentationList/>
        <micrographsFilePattern>data/EMPIAR_sjojoa/gains/*mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
