Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with progressive supranuclear palsy

REL 2021-07-07 2021-09-17 2021-10-19 Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with progressive supranuclear palsy 0000-0003-1579-7561 Yang Shi MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Sjors HW Scheres MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Michel Goedert MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Shi Y Zhang W Yang Y Murzin AG Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DMA Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SHW 967.4 10.6019/EMPIAR-10765 EMDB EMD-13218 Shi Y Zhang W Yang Y Murzin AG Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DMA Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SHW Structure-based classification of tauopathies Nature Nature 7880 598 359 363 2021 English 10.1038/s41586-021-03911-7 34588692 Shi Y Zhang W Yang Y Murzin A Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DM Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SH Structure-based Classification of Tauopathies bioRxiv 2021 10.1101/2021.05.28.446130 Unaligned multi-frame movies /data/PSP/Movies micrographs - multiframe TIFF TIFF 6601 40 UNSIGNED BYTE 3838 1.15 3710 1.15

The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.0155 e/A2/frame. Please use Movies/gain.mrc as a gain reference without any rotation or flipping. data/PSP/Start_end_coordinates contains start-end coordinates of the manually picked filaments. data/PSP/Data.star contains STAR file with the metadata of the final refined segments.

data/PSP/Movies/Images-Disc1/GridSquare_*/Data/*.tif data/PSP/Start_end_coordinates/Movies/Images-Disc1/GridSquare_*/Data/*_manualpick.star data/PSP/Start_end_coordinates