Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the nucleus accumbens of an individual with argyrophilic grain disease

REL 2021-07-17 2021-09-17 2021-10-19 Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the nucleus accumbens of an individual with argyrophilic grain disease 0000-0003-1579-7561 Yang Shi MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Sjors HW Scheres MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Michel Goedert MRC-LMB Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Shi Y Zhang W Yang Y Murzin AG Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DMA Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SHW 2.8 10.6019/EMPIAR-10768 EMDB EMD-13226 Shi Y Zhang W Yang Y Murzin AG Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DMA Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SHW Structure-based classification of tauopathies Nature Nature 7880 598 359 363 2021 English 10.1038/s41586-021-03911-7 34588692 Shi Y Zhang W Yang Y Murzin A Falcon B Kotecha A van Beers M Tarutani A Kametani F Garringer HJ Vidal R Hallinan GI Lashley T Saito Y Murayama S Yoshida M Tanaka H Kakita A Ikeuchi T Robinson AC Mann DM Kovacs GG Revesz T Ghetti B Hasegawa M Goedert M Scheres SH Structure-based Classification of Tauopathies bioRxiv 2021 10.1101/2021.05.28.446130 Unaligned multi-frame movies /data/AGD/Movies micrographs - multiframe TIFF TIFF 15227 35 UNSIGNED BYTE 5760 1.17 4092 1.17

The files were written as gain-unnormalised TIF files by EPU. The dose rate is 0.89 e/A2/frame. Please use Movies/FoilHole_19945800_Data_19967971_19967973_20210320_192134_gain.tiff as a gain reference without any rotation or flipping. data/AGD/start_end_coordinates contains start-end coordinates of the manually picked filaments. data/AGD/data.star contains STAR files with the metadata of the final refined segments.

data/AGD/Movies/M*/Images-Disc1/GridSquare*/Data/*fractions.tiff data/AGD/start_end_coordinates/M*/Images-Disc1/GridSquare*/Data/*manualpick.star data/AGD/start_end_coordinates