REL
2021-07-24
2021-09-10
2022-02-25
cryo electron tomography of Apoferritin
0000-0001-7268-0306
Tao
Ni
Division of Structural Biology, University of Oxford
Roosevelt Drive
Oxford
Oxfordshire
United Kingdom
OX 3 7BN
0000-0003-1803-691X
Peijun
Zhang
Division of Structural Biology, University of Oxford
Roosevelt Drive
Oxford
Oxfordshire
United Kingdom
OX3 7BN
Ni T
Frosio T
Mendonça L
Sheng Y
Clare D
Himes BA
Zhang P
17.3
10.6019/EMPIAR-10787
EMDB
EMD-13271
Ni T
Frosio T
Mendonça L
Sheng Y
Clare D
Himes BA
Zhang P
High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity
Nature protocols
Nat Protoc
2
17
421
444
2022
English
10.1038/s41596-021-00648-5
35022621
The micrographs are colllected with Gatan K3 camera, in super-resolution mode. Energy filter 20 eV, Dose rate 4.2 electron/A2/s, Total dose 102 electrons, 10 frames in raw tiff images, Pixel size 1.34 angstrom
Unaligned raw tilt-series of apoferritin on graphene grid
/data
tilt series
TIFF
TIFF
6
10
1
10
4 BIT INTEGER
11520
0.67
8184
0.67
Raw tiff images, collected in serialEM software, dose-symmetric scheme, group of 2.