REL 2021-09-28 2021-10-25 2022-02-25 0000-0002-7011-7234 Laura van Niftrik Microbial Cell Biology and Biochemistry, Dept. Microbiology, RIBES institute, Faculty of Science, Radboud University Heyenaalseweg 135 Nijmegen Netherlands 6525 AJ 0000-0002-2257-6364 Lavinia Gambelli Living Systems Institute, University of Exeter Stocker Road Exeter United Kingdom EX4 4QD 0000-0003-0877-3676 Rob Mesman Microbial Cell Biology and Biochemistry, Dept. Microbiology, RIBES institute, Faculty of Science, Radboud University Heyenaalseweg 135 Nijmegen Netherlands 6525 AJ 0000-0002-7011-7234 Laura van Niftrik Microbial Cell Biology and Biochemistry, Dept. Microbiology, RIBES institute, Faculty of Science, Radboud University Heyenaalseweg 135 Nijmegen Netherlands 6525 AJ Gambelli L Mesman R Versantvoort W Diebolder CA Engel A Evers W Jetten MSM Pabst M Daum B van Niftrik L 46.3 10.6019/EMPIAR-10822 EMDB EMD-13670 Gambelli L Mesman R Versantvoort W Diebolder CA Engel A Evers W Jetten MSM Pabst M Daum B van Niftrik L Frontiers in microbiology Front Microbiol 12 2021 English 10.3389/fmicb.2021.766527 34925275

Tomograms were reconstructed from aligned movie frames (10 frames per tilt angle, exposure dose 0.17154 e/A^2 per frame, total dose ~103 e/A^2 for the whole tilt-series) Details on the acquisition can be found in the .mdoc file, tilt-angles are documented in the .tlt file.

Reconstructed tomogram of isolated Ca. M.lanthanidiphila S-layer patches /data/Mlanthanidiphila_Slayer31 reconstructed volumes MRC MRC 672 10 SIGNED 16 BIT INTEGER 4092 2.62631 5760 2.62631

Aquisition parameters in the corresponding mdoc file. Tilt-angles for missing wedge compensation in the corresponding tlt file

Reconstructed tomogram of isolated Ca. M.lanthanidiphila S-layer patches /data/Mlanthanidiphila_Slayer32 reconstructed volumes MRC MRC 382 10 SIGNED 16 BIT INTEGER 4092 2.62631 5760 2.62631

Aquisition parameters in the corresponding mdoc file. Tilt-angles for missing wedge compensation in the corresponding tlt file