Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments

REL 2021-11-01 2022-01-25 2023-01-23 Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments 0000-0003-2238-6437 Yang Yang LMB MRC-LMB Cambridge UK United Kingdom CB2 0QH 0000-0002-1842-8019 Bernardino Ghetti Department of Pathology and Laboratory Medicine, Indiana University, Indianapolis IN United States 46202 0000-0002-8176-2618 Benjamin Falcon MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge United Kingdom CB2 0QH 0000-0002-0462-6540 Sjors HW Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge United Kingdom CB2 0QH 0000-0002-5214-7886 Michel Goedert MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge United Kingdom CB2 0QH Yang Y Arseni D Zhang WJ Huang M Lovestam SKA Schweighauser M Kotecha A Murzin AG Peak-Chew S Macdonald J Lavenir I Garringer HJ Gelpi E Newell KL Kovacs GG Vidal R Ghetti B Falcon B Scheres SHW Goedert M 531.9 10.6019/EMPIAR-10916 EMDB molecule EMD-13800 Yang Y Arseni D Zhang WJ Huang M Lovestam SKA Schweighauser M Kotecha A Murzin AG Peak-Chew S Macdonald J Lavenir I Garringer HJ Gelpi E Newell KL Kovacs GG Vidal R Ghetti B Falcon B Scheres SHW Goedert M Cryo-EM structures of amyloid-β 42 filaments from human brains Science (New York, N.Y.) Science United States 6577 375 167 172 2022 English 10.1126/science.abm7285 35025654

The files were written as eer files by EPU and then converted to tiff files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame. Please use gain.mrc as a gain reference without any rotation or flipping. start_end_coordinates contains start-end coordinates of the manually picked filaments. data.star contains STAR file(s) with the metadata of the final refined segments. Processing details are in the folder AD_case1_processing.

Yang Y Arseni D Zhang WJ Huang M Lövestam S Schweighauser M Kotecha A Murzin AG Peak-Chew SY Macdonald J Lavenir I Garringer HJ Gelpi E Newell KL Kovacs GG Vidal R Ghetti B Falcon B Scheres SHW Goedert M Cryo-EM Structures of Amyloid-β 42 Filaments from Human Brain bioRxiv 2021 English 10.1101/2021.10.19.464936 Unaligned multi-frame movies /data/AD1/Micrographs/TIFF micrographs - multiframe TIFF TIFF 1762 40 UNSIGNED BYTE 4096 0.727 4096 0.727

The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame. Please use gain-reference.mrc as a gain reference without any rotation or flipping. pick_coordinates contains start-end coordinates of the manually picked filaments. Refine097_run_data.star contains STAR file(s) with the metadata of the final refined segments.