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        <keyDates>
            <depositionDate>2022-02-03</depositionDate>
            <releaseDate>2022-03-08</releaseDate>
            <updateDate>2022-03-28</updateDate>
        </keyDates>
        <title>Tutorial for high-throughput cryo-EM structure determination of amyloids: Dataset 2</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-0462-6540</authorORCID>
            <firstName>Sjors</firstName>
            <middleName>H W</middleName>
            <lastName>Scheres</lastName>
            <organization type="academic">MRC Lab of Molecular Biology</organization>
            <townOrCity>Cambridge</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>CB20QH</postOrZipCode>
        </correspondingAuthor>
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            <authorORCID>0000-0002-0462-6540</authorORCID>
            <firstName>Sjors</firstName>
            <middleName>H W</middleName>
            <lastName>Scheres</lastName>
            <organization type="academic">MRC Lab of Molecular Biology</organization>
            <townOrCity>Cambridge</townOrCity>
            <country>United Kingdom</country>
            <postOrZipCode>CB20QH</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-2152-1476">Lövestam S</author>
            <author authorORCID="0000-0002-0462-6540">Scheres SHW</author>
        </authorsList>
        <datasetSize units="TB">1.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-10943</entryDOI>
        <experimentType>EMDB</experimentType>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-14038</emdbEntry>
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                    <author authorORCID="0000-0002-2152-1476" order="1">Lövestam S</author>
                    <author order="2">Koh FA</author>
                    <author order="3">van Knippenberg B</author>
                    <author order="4">Kotecha A</author>
                    <author order="5">Murzin AG</author>
                    <author order="6">Goedert M</author>
                    <author authorORCID="0000-0002-0462-6540" order="7">Scheres SHW</author>
                    <title>Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy</title>
                    <journal>eLife</journal>
                    <journalAbbreviation>Elife</journalAbbreviation>
                    <country></country>
                    <volume>11</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.7554/elife.76494</externalReferences>
                    <externalReferences type="pubmed">35244536</externalReferences>
                    <details>The micrographs and processing of three amyloid datasets. This is dataset 2.</details>
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    <imageSet>
        <name>Unaligned multi-frame movies in Tiff: Dataset 2</name>
        <directory>/data/Tiff</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4000</numImagesOrTiltSeries>
        <framesPerImage>34</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>0.824</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>0.824</pixelHeight>
        </dimensions>
        <details>Movies were collected in EER format and converted into tiff using relion_convert_to_tiff grouping frames by 34 giving a dose of 0.98 e/Å^2. Please see additional files for complete processing pipeline.</details>
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