REL 2022-02-25 2022-03-11 2022-10-17 0000-0002-1848-1610 Manuel Schweighauser MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0002-5214-7886 Michel Goedert MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB1 0QH 0000-0002-0462-6540 Sjors HW Scheres MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Schweighauser M Arseni D Bacioglu M Huang M Lövestam S Shi Y Yang Y Zhang W Kotecha A Garringer HJ Vidal R Hallinan GI Newell KL Tarutani A Murayama S Miyazaki M Saito Y Yoshida M Hasegawa K Lashley T Revesz T Kovacs GG van Swieten J Takao M Hasegawa M Ghetti B Spillantini MG Ryskeldi-Falcon B Murzin AG Goedert M Scheres SHW 1.0 10.6019/EMPIAR-10968 EMDB molecule EMD-14176 Schweighauser M Arseni D Bacioglu M Huang M Lövestam S Shi Y Yang Y Zhang W Kotecha A Garringer HJ Vidal R Hallinan GI Newell KL Tarutani A Murayama S Miyazaki M Saito Y Yoshida M Hasegawa K Lashley T Revesz T Kovacs GG van Swieten J Takao M Hasegawa M Ghetti B Spillantini MG Ryskeldi-Falcon B Murzin AG Goedert M Scheres SHW Nature Nature 7909 605 310 314 2022 English 10.1038/s41586-022-04650-z 35344985 Unaligned multi-frame movies /data/TMEM106B_Case18/Movies micrographs - multiframe TIFF TIFF 5697 54 UNSIGNED BYTE 3838 1.15 3710 1.15

Movies were recorded automatically using EPU on a K2 camera in counting mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rates are 0.91 and 0.89 e/A2/frame for Dataset1 and Dataset2, respectively. The gain references are provided for each dataset and should be used without any rotation or flipping. data/TMEM106B_Case18/start_end_coordinates contains start-end coordinates of the manually picked filaments from datasets 1 and 2. data/TMEM106B_Case18/run_data_TMEM10B_Case18_job534.star is the RELION-3.1 STAR file containing the metadata of the final refined segments.