REL 2022-02-17 2022-10-07 2022-10-07 0000-0003-1813-7008 Osamu Nureki Department of Biological Sciences Graduate School of Science The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo Japan 113-0033 0000-0003-1813-7008 Osamu Nureki Department of Biological Sciences Graduate School of Science The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 7-3-1 Hongo Bunkyo-ku Tokyo Japan 113-0033 0000-0002-1941-5535 Hideaki E Kato Komaba Institute for Science, The University of Tokyo Tokyo Japan 153-0041 Kobayashi K Kawakami K Kusakizako T Miyauchi H Tomita A Kobayashi K Shihoya W Yamashita K Nishizawa T Kato HE Inoue A Nureki O 3.8 10.6019/EMPIAR-10996 EMDB EMD-32141 EMD-32142 EMD-32143 EMD-32144 EMD-32145 EMD-32146 Kobayashi K Kawakami K Kusakizako T Miyauchi H Tomita A Kobayashi K Shihoya W Yamashita K Nishizawa T Kato HE Inoue A Nureki O Japan Unaligned movies for the PTH bound human PTH1R-Gs complex /data/Movies/Dataset1 micrographs - multiframe TIFF TIFF 6205 54 1 54 UNSIGNED BYTE 5760 0.83 4092 0.83

Gain reference is provided, which need to be flipped (‘Flip upside down (1)’ in RELION).

data/Movies/Dataset1/*.tif data/Particles/dataset1_all.star data/Particles/Dataset1 Unaligned movies for the PTHrP bound human PTH1R-Gs complex /data/Movies/Dataset2 micrographs - multiframe TIFF TIFF 9405 48 1 48 UNSIGNED BYTE 5760 0.83 4092 0.83

Gain reference is provided, which need to be flipped (‘Flip upside down (1)’ in RELION).

data/Movies/Dataset2/*.tif data/Particles/dataset2-all.star data/Particles/Dataset2