REL 2021-04-07 2025-04-07 2025-04-07 0000-0001-9160-1941 Peter Sadler Department of Chemistry, University of Warwick Gibbet Hill Road Coventry Warwickshire United Kingdom CV47AL 0000-0002-7607-4271 Paul David Quinn Diamond Light Source Fermi Avenue Didcot Oxfordshire United Kingdom OX11 0DE 0000-0003-1187-7951 Cinzia Imberti Department of Chemistry, University of Warwick Gibbet Hill Road Coventry Warwickshire United Kingdom CV47AL 0000-0001-8091-9057 Maria Harkiolaki Diamond Light Source Fermi Avenue Didcot Oxfordshire United Kingdom OX11 0DE Bolitho EM Sanchez-Cano C Shi H 43.9 10.6019/EMPIAR-11044 SXT Bolitho EM Sanchez-Cano C Shi H Quinn PD Harkiolaki M Imberti C Sadler PJ Journal of the American Chemical Society J Am Chem Soc United States 48 143 20224 20240 2021 English 10.1021/jacs.1c08630 34808054 Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 1 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 15.8 946 15.8

Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 2 (T2) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 2 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 2 (T2) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 2 (T2) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 2 tilt series TIFF TIFF 1 651 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 2 (T2) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 1 tilt series TIFF TIFF 1 281 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 3 (T3) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 3 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 3 (T3) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 3 (T3) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Tomogram 3 tilt series TIFF TIFF 1 626 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 3 (T3) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h.

Tomogram 4 (T4) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 4 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 4 (T4) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 4 (T4) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 4 tilt series TIFF TIFF 1 261 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 4 (T4) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 5 (T5) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 5 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 5 (T5) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 5 (T5) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 5 tilt series TIFF TIFF 1 651 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 5 (T5) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 6 (T6) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 6 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 6 (T6) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 6 (T6) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm) /data/Tomogram 6 tilt series TIFF TIFF 1 281 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 6 (T6)- Reconstructed tomogram of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to 1 h irradiation (blue light, λ=465 nm).

Tomogram 7 (T7) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark) tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 7 (T7) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 7 (T7) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark)/Tomogram 7 tilt series TIFF TIFF 1 241 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 7 (T7) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h /data/Untreated Control (Dark)/Tomogram 1 tilt series TIFF TIFF 1 281 UNSIGNED 16 BIT INTEGER 946 variable 946 variable

Tomogram 1 (T1) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids exposed to dark conditions for 2 h

Tomogram 8 (T8) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark)/Tomogram 8 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 8 (T8) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 8 (T8) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark)/Tomogram 8 tilt series TIFF TIFF 1 261 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 8 (T8) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 9 (T9) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark)/Tomogram 9 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 9 (T9) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 9 (T9) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light) /data/1x IC50 of Pt2 (Dark)/Tomogram 9 tilt series TIFF TIFF 1 261 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 9 (T9) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 2 h (protected from light)

Tomogram 10 (T10) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 10 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 10 (T10) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 10 (T10) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 10 tilt series TIFF TIFF 1 270 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 10 (T10) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 11 (T11) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 11 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 11 (T11) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 11 (T11) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 11 tilt series TIFF TIFF 1 271 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 11 (T11) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 12 (T12) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 12 tilt series TIFF TIFF 1 676 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 12 (T12) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 12 (T12) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.25x IC50 of Pt2 (Light)/Tomogram 12 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 12 (T12) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 13 (T13) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.5x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.5x IC50 of Pt2 (Light)/Tomogram 13 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 13 (T13) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 13 (T13) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.5x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.5x IC50 of Pt2 (Light)/Tomogram 13 tilt series TIFF TIFF 1 271 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 13 (T13) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.25x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 14 (T14) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.5x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.5x IC50 of Pt2 (Light)/Tomogram 14 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 14 (T14) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.55x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 14 (T14) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.5x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/0.5x IC50 of Pt2 (Light)/Tomogram 14 tilt series TIFF TIFF 1 576 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 14 (T14) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 0.5x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 15 (T15) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 15 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 15 (T15) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 15 (T15) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 15 tilt series TIFF TIFF 1 231 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 15 (T15) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 16 (T16) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 16 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 16 (T16) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 16 (T16) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 16 tilt series TIFF TIFF 1 601 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 16 (T16) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 17 (T17) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 17 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 17 (T17) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 17 (T17) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) /data/1x IC50 of Pt2 (Light)/Tomogram 17 tilt series TIFF TIFF 1 651 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 17 (T17) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm)

Tomogram 18 (T18) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery /data/1x IC50 of Pt2 (Light) + 2 h recovery/Tomogram 18 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 18 (T18) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in dru free media

Tomogram 18 (T18) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery /data/1x IC50 of Pt2 (Light) + 2 h recovery/Tomogram 18 tilt series TIFF TIFF 1 211 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 18 (T18) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in drug free media.

Tomogram 19 (T19) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in /data/1x IC50 of Pt2 (Light) + 2 h recovery/Tomogram 19 tilt series TIFF TIFF 1 651 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 19 (T19) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in drug free media

Tomogram 19 (T19) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in /data/1x IC50 of Pt2 (Light) + 2 h recovery/Tomogram 19 tilt series MRC MRC 1 1 UNSIGNED 16 BIT INTEGER 946 158.0 946 158.0

Tomogram 19 (T19) - Tilt series of cryopreserved PC3 cell grown on Quantifoil TEM grids treated with 1x photo IC50 of Pt2 for 1 h followed by 1 irradiation (blue light, λ=465 nm) and 2 h recovery in drug free media