REL
2020-12-29
2022-06-07
2022-06-07
Cryo-EM Structure of the Hyperpolarization-Activated Potassium Channel KAT1
0000-0001-9782-6682
Michael
Clark
University of Chicago: Biochemistry and Molecular Biology Department
Chicago
Illinois
United States
60637
0000-0001-9782-6682
Michael
Clark
University of Chicago: Biochemistry and Molecular Biology Department
Chicago
Illinois
United States
60637
Clark MD
Contreras GF
Shen R
Perozo E
3.1
10.6019/EMPIAR-11054
EMDB
EMD-21019
EMD-21018
Clark MD
Contreras GF
Shen R
Perozo E
Electromechanical coupling in the hyperpolarization-activated K<sup>+</sup> channel KAT1
Nature
Nature
7814
583
145
149
2020
English
10.1038/s41586-020-2335-4
32461693
Movies were collected on a Titan Krios with K2 detector (super-resolution mode) and GIF energy filter (set to 20 eV) at a nominal magnification of 130,000 corresponding to a super-resolution pixel size 0.532 Å per pixel. The dose rate was roughly 4.7 e− pixel−1 s−1 and the exposure time was 12 s, yielding a total post-GIF dose of 38–43 e− Å−2. A total of 1,502 movies were collected, with 40 frames saved per movie. Deposited are non-gain-referenced movies.
unaligned non-gain-ref movies
/data/movies
micrographs - multiframe
MRC
MRC
1503
40
UNSIGNED BYTE
variable
variable
variable
variable
Gain refererence file is included.