Structure of bile acid transporter NTCP

REL 2022-05-18 2022-06-13 2022-06-13 Structure of bile acid transporter NTCP 0000-0002-4422-4044 Umeharu Ohto Graduate School of Pharmaceutical Sciences, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 0000-0002-4422-4044 Umeharu Ohto Graduate School of Pharmaceutical Sciences, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 Asami J Shimizu T Ohto U 8.6 10.6019/EMPIAR-11056 EMDB molecule EMD-31837 EMD-31838 EMD-31839 EMD-31840 EMD-32759 Asami J Kimura KT Fujita-Fujiharu Y Ishida H Zhang Z Nomura Y Liu K Uemura T Sato Y Ono M Yamamoto M Noda T Shigematsu H Drew D Iwata S Shimizu T Nomura N Ohto U Structure of bile acid transporter NTCP crucial for hepatitis B virus entry Nature Nature 2022 English 10.1038/s41586-022-04845-4 35580629 Raw movies for Human NTCP (Q261A) - YN69202Fab (EMD-31837, PDB 7VAD) /data/Data1_PDB_7VAD_EMD_31837/20210514_shimizulab_210513_15_J1_JA/Micrographs micrographs - multiframe TIFF TIFF 5434 64 1 64 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 0.95 e/Å2/sec Please use CountCDSRef_ja_115-1_0007_May14_21.29.53.mrc as a gain reference without rotation and flipping. Four digits in the file names (0000 to 0008) represent beam shift directions.

Raw movies for Bovine NTCP (Q261A) - YN69202Fab (EMD-31838, PDB 7VAE) /data/Data2_PDB_7VAE_EMD_31838/20210428_shimizulab_210427_2_H2_JA/Micrographs micrographs - multiframe TIFF TIFF 2712 64 1 64 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 0.95 e/Å2/sec Please use CountCDSRef_ja_92-1_0000_Apr28_20.18.32.mrc as a gain reference without rotation and flipping. Four digits in the file names (0000 to 0008) represent beam shift directions.

Raw movies for Rat NTCP (Q261A) - YN69202Fab (EMD-31839, PDB 7VAE) /data/Data3_PDB_7VAF_EMD_31839 micrographs - multiframe TIFF TIFF 9932 64 1 64 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 0.95 e/Å2/sec This structure was solved from datasets collected in three sessions. Please use CountCDSRef_ja_73-1_0000_Apr15_19.29.35.mrc (for 20210415_shimizulab_210414_2_P2_JA), CountCDSRef_ja_119-1_0000_Apr29_11.04.43.mrc (for 20210429_shimizulab_210427_1_H1_JA) and CountCDSRef_ja_111-1_0000_Apr30_22.44.30.mrc (for 20210430_shimizulab_210430_9_E3_JA) as a gain reference without rotation and flipping. Four digits in the file names (0000 to 0008) represent beam shift directions.

Raw movies for Human NTCP (Q261A) - YN69202Fab-myr-preS1 (EMD-31840, PDB 7VAF) /data/Data4_PDB_7VAG_EMD_31840 micrographs - multiframe TIFF TIFF 7496 64 1 64 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 0.95 e/Å2/sec This structure was solved from datasets collected in two sessions. Please use CountCDSRef_ja_147-1_0000_Jul22_19.18.51.mrc (for 20210722_shimizulab_210720_4_F4_JA) and CountCDSRef_ja_159-1_0000_Aug06_15.07.36.mrc (for 20210806_shimizulab_210804_1_B1_JA) as a gain reference without rotation and flipping. Four digits in the file names (0000 to 0008) represent beam shift directions.

Raw movies for Human NTCP (wildtype) - YN69202Fab (EMD-32759, PDB 7WSI) /data/Data5_PDB_7WSI_EMD_32759/20211218_shimizulab_211217_6_J2_JA/Micrographs micrographs - multiframe TIFF TIFF 3411 64 1 64 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 0.95 e/Å2/sec Please use CountCDSRef_ja_125-1_0000_Dec18_16.34.47.mrc as a gain reference without rotation and flipping. Four digits in the file names (0000 to 0008) represent beam shift directions.