PMCA-amplified α-synuclein fibrils, Multiple System Atrophy patient-derived seeds

REL 2021-06-29 2022-07-27 2022-07-27 PMCA-amplified α-synuclein fibrils, Multiple System Atrophy patient-derived seeds 0000-0003-3378-0683 James Alexander Geraets Forschungszentrum Jülich Jülich Germany 52425 0000-0003-1803-5431 Gunnar F Schröder Forschungszentrum Jülich Jülich Germany 52425 Frieg B Geraets JA Schröder GF 392.8 10.6019/EMPIAR-11095 EMDB molecule EMD-13124 Frieg B Geraets JA Strohäker T Dienemann C Mavroeidi P Jung BC Kim WS Lee SJ Xilouri M Zweckstetter M Schröder GF Quaternary structure of patient-homogenate amplified α-synuclein fibrils modulates seeding of endogenous α-synuclein Aligned averages of PMCA-amplified α-synuclein fibrils, Multiple System Atrophy patient-derived seeds /data/Micrographs micrographs - single frame MRC MRC 4474 1 32 BIT FLOAT 5760 1.05 4092 1.05

Provided are aligned average micrographs collected on a Titan Krios at 300kV with a K3 detector operating in non-superresolution counting mode. Applied defocus values ranged from −0.7 to −2.9 µm. MotionCor2 was used to align 40 dose fractions that were taken with a total electron dose of ~43.1e-/Å². Coordinates provided are of start-end pairs, for helical segmentation and extraction.

data/Micrographs/*.mrc data/Coordinates/*_manualpick.star data/Coordinates/