Cryo-EM structure of Cas13bt3–guide RNA

REL 2022-06-28 2022-09-20 2022-09-20 Cryo-EM structure of Cas13bt3–guide RNA–target RNA complex 0000-0002-6186-6647 Tsukasa Kusakizako Department of Biological Sciences, Graduate School of Science, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 0000-0002-6186-6647 Osamu Nureki Department of Biological Sciences, Graduate School of Science, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 Nakagawa R Kannan S Altae-Tran H Takeda SN Tomita A Hirano H Kusakizako T Nishizawa T Yamashita K Zhang F Nishimasu H Nureki O 616.9 10.6019/EMPIAR-11110 EMDB molecule EMD-32118 Nakagawa R Kannan S Altae-Tran H Takeda SN Tomita A Hirano H Kusakizako T Nishizawa T Yamashita K Zhang F Nishimasu H Nureki O Structure and engineering of the minimal type VI CRISPR-Cas13bt3 Molecular cell Mol Cell 17 82 3178 3192.e5 2022 English 10.1016/j.molcel.2022.08.001 36027912 Unaligned movies for Cas13bt3–guide RNA–target RNA complex /data/20200601_Nureki_nakagawa4_data/movies micrographs - multiframe TIFF TIFF 2772 48 1 48 UNSIGNED BYTE 5760 0.83 4092 0.83

HT: 300 kV Cs: 2.7 mm Flux: 1 e/Å2/frame Please use 'CountRef_nakagawa4_80-1_0000_Jun01_11.35.15.mrc' as a gain reference without rotation or flipping. The calibrated pixel size is 0.83 Å, while the nominal pixel size of 0.85 Å is shown in the tiff header.