REL
2022-06-28
2022-11-11
2022-11-11
Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the cingulate cortex of an individual with Parkinson's disease dementia -synuclein filaments
0000-0003-2238-6437
Yang
Yang
MRC-LMB
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-0462-6540
Sjors
HW
Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0002-5214-7886
Michel
Goedert
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Yang Y
Shi Y
Schweighauser M
Zhang X
Kotecha A
Murzin AG
Garringer HJ
Cullinane PW
Saito Y
Foroud T
Warner TT
Hasegawa K
Vidal R
Murayama S
Revesz T
Ghetti B
Hasegawa M
Lashley T
Scheres SHW
Goedert M
1.2
10.6019/EMPIAR-11140
EMDB
molecule
EMD-15285
Yang Y
Shi Y
Schweighauser M
Zhang X
Kotecha A
Murzin AG
Garringer HJ
Cullinane PW
Saito Y
Foroud T
Warner TT
Hasegawa K
Vidal R
Murayama S
Revesz T
Ghetti B
Hasegawa M
Lashley T
Scheres SHW
Goedert M
Structures of α-synuclein filaments from human brains with Lewy pathology
Nature
Nature
7933
610
791
795
2022
English
10.1038/s41586-022-05319-3
36108674
Yang Y
Shi Y
Schweighauser M
Zhang X
Kotecha A
Murzin AG
Garringer HJ
Cullinane PW
Saito Y
Foroud T
Warner TT
Hasegawa K
Vidal R
Murayama S
Revesz T
Ghetti B
Hasegawa M
Lashley T
Scheres SHW
Goedert M
Cryo-EM structures of α-synuclein filaments from Parkinson’s disease and dementia with Lewy bodies
bioRxiv
2022
10.1101/2022.07.12.499706
Cryo-EM reconstruction of alpha-synuclein filaments from Parkinson's disease dementia
/data/Micrographs
micrographs - multiframe
TIFF
TIFF
10874
42
UNSIGNED BYTE
4096
0.727
4096
0.727
The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame for Dataset1 and Dataset2. Please use gain-reference accordingly without any rotation or flipping.
picking contains start-end coordinates of the manually picked filaments of Dataset1 and Dataset2, respectively.
Refine070_run_data.star contains STAR file(s) with the metadata of the final refined segments.
data/Micrographs/Dataset*
data/picking/Dataset*
data