<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11168" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-08-16</depositionDate>
            <releaseDate>2022-09-09</releaseDate>
            <updateDate>2022-09-23</updateDate>
        </keyDates>
        <title>Structure of SARS-CoV-2 membrane protein</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-4422-4044</authorORCID>
            <firstName>Umeharu</firstName>
            <lastName>Ohto</lastName>
            <organization type="academic">Graduate School of Pharmaceutical Sciences, The University of Tokyo</organization>
            <street>7-3-1, Hongo</street>
            <townOrCity>Bunkyo</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-4422-4044</authorORCID>
            <firstName>Umeharu</firstName>
            <lastName>Ohto</lastName>
            <organization type="academic">Graduate School of Pharmaceutical Sciences, The University of Tokyo</organization>
            <street>7-3-1, Hongo</street>
            <townOrCity>Bunkyo</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Zhang Z</author>
            <author authorORCID="0000-0002-4422-4044">Ohto U</author>
            <author authorORCID="0000-0002-0547-8922">Shimizu T</author>
        </authorsList>
        <datasetSize units="TB">4.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-11168</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-31977</emdbEntry>
            <emdbEntry>EMD-31976</emdbEntry>
            <emdbEntry>EMD-31978</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Zhang Z</author>
                    <author authorORCID="0000-0002-6330-2239" order="2">Nomura N</author>
                    <author authorORCID="0000-0002-8706-3113" order="3">Muramoto Y</author>
                    <author order="4">Ekimoto T</author>
                    <author order="5">Uemura T</author>
                    <author order="6">Liu K</author>
                    <author order="7">Yui M</author>
                    <author authorORCID="0000-0002-0871-8477" order="8">Kono N</author>
                    <author order="9">Aoki J</author>
                    <author order="10">Ikeguchi M</author>
                    <author order="11">Noda T</author>
                    <author order="12">Iwata S</author>
                    <author authorORCID="0000-0002-4422-4044" order="13">Ohto U</author>
                    <author authorORCID="0000-0002-0547-8922" order="14">Shimizu T</author>
                    <title>Structure of SARS-CoV-2 membrane protein essential for virus assembly</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>13</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-022-32019-3</externalReferences>
                    <externalReferences type="pubmed">35931673</externalReferences>
                    <details>This entry contains raw movies of three datasets: M protein without Fab, M protein with Fab-E and M protein with Fab-B.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>M protein (LMNG/CHS)</name>
        <directory>/data/20210107_shimizulab_210106_4_P4_ZZ/Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6055</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>60</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>HT: 300 kV
Cs: 2.7 mm
Flux: 0.96 e/Å2/frame
Please use 'CountRef_112-1_0000_Jan07_20.02.08.mrc' as a gain reference without rotation or flipping.
NB: The pixel size in the TIFF header is not correct.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>M protein (LMNG/CHS) + Fab-E</name>
        <directory>/data/20210521_shimizulab_210520_22_P2_ZZ/Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5562</numImagesOrTiltSeries>
        <framesPerImage>64</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>64</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>HT: 300 kV
Cs: 2.7 mm
Flux: 0.97 e/Å2/frame
Please use 'CountCDSRef_ja_139-1_0000_May21_22.27.44.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>M protein (LMNG/CHS) + Fab-B</name>
        <directory>/data/20210527_shimizulab_210520_19_B3_ZZ/Micrographs</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3519</numImagesOrTiltSeries>
        <framesPerImage>64</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>64</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>HT: 300 kV
Cs: 2.7 mm
Flux: 0.96 e/Å2/frame
Please use 'CountCDSRef_ja_145-1_0000_May27_23.14.16.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the movie file names represent the directions of beam shifts applied during data collection.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
