REL 2022-08-18 2022-09-09 2022-09-09 0000-0001-6164-8896 Sam-Yong Park Graduate School of Medical Life Science, Yokohama City University 1-7-29, Suehiro, Tsurumi Yokohama Kanagawa Japan 230-0045 0000-0001-6164-8896 Sam-Yong Park Graduate School of Medical Life Science, Yokohama City University 1-7-29, Suehiro, Tsurumi Yokohama Kanagawa Japan 230-0045 Park JH Iwamoto M Yun JH Uchikubo-Kamo T Son D Jin Z Yoshida H Ohki M Ishimoto N Mizutani K Oshima M Muramatsu M Wakita T Shirouzu M Liu K Uemura T Nomura N Iwata S Watashi K Tame JRH Nishizawa T Lee W Park SY 5.5 10.6019/EMPIAR-11178 EMDB molecule EMD-31526 Park JH Iwamoto M Yun JH Uchikubo-Kamo T Son D Jin Z Yoshida H Ohki M Ishimoto N Mizutani K Oshima M Muramatsu M Wakita T Shirouzu M Liu K Uemura T Nomura N Iwata S Watashi K Tame JRH Nishizawa T Lee W Park SY Nature Nature 7916 606 1027 1031 2022 English 10.1038/s41586-022-04857-0 35580630

This entry contains raw movies for human NTCP in complex with YN69083 Fab.

Unaligned, non-dose weighted movies of NTCP-YN69083Fab Data 1 /data/Dataset1 micrographs - multiframe TIFF TIFF 5845 49 1 49 UNSIGNED BYTE 5760 0.829 4092 0.829

HT: 300 kV Cs: 2.7 mm Flux: 50 e/Å2 in 49 frames 'FoilHole_18713532_Data_18692370_18692372_20210120_003013_GainReference.tiff' is a super-resolution gain reference written by EPU. You have to downscale it by 2 with harmonic mean. This can be done with EMAN2 as: e2proc2d.py --process math.reciprocal --meanshrink 2 --process math.reciprocal FoilHole_18713532_Data_18692370_18692372_20210120_003013_GainReference.tiff dataset1-gain.mrc The gain does not need any rotation or flipping. The dataset was collected with AFIS. AFIS directions are available in XMLs files, although grouping was not applied during processing. Unfortunately, an XML file for FoilHole_18729909_Data_18692370_18692372_20210120_104541_Fractions.tiff was lost. NB: The pixel size in the TIFF header is not correct. Although the paper says 5846 movies, one of them was corrupted, so only 5845 were used for processing.

Unaligned, non-dose weighted movies of NTCP-YN69083Fab Data 2 /data/Dataset2 micrographs - multiframe TIFF TIFF 15121 65 1 65 UNSIGNED BYTE 5760 0.829 4092 0.829

HT: 300 kV Cs: 2.7 mm Flux: 64 e/Å2 in 65 frames 'FoilHole_18796351_Data_18756374_18756376_20210220_200533_gain.tiff' is a super-resolution gain reference written by EPU. You have to downscale it by 2 with harmonic mean. This can be done with EMAN2 as: e2proc2d.py --process math.reciprocal --meanshrink 2 --process math.reciprocal FoilHole_18796351_Data_18756374_18756376_20210220_200533_gain.tiff dataset2-gain.mrc The gain does not need any rotation or flipping. Please exclude the first frame from motion correction and Polishing, because it is corrupted due to glitches in the K3 CDS mode. The dataset was collected with AFIS. AFIS directions are available in XMLs files, although grouping was not applied during processing. Unfortunately, XML files for FoilHole_18775720_Data_18756374_18756376_20210220_023647_fractions.tiff and FoilHole_18816258_Data_18756374_18756376_20210221_102409_fractions.tiff were lost. NB: The pixel size in the TIFF header is not correct.