REL 2022-06-28 2023-04-14 2023-04-14 Thermostabilized human prestin in complex with chloride, sulfate or salicylate 0000-0002-6186-6647 Tsukasa Kusakizako Department of Biological Sciences, Graduate School of Science, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 0000-0002-6186-6647 Osamu Nureki Department of Biological Sciences, Graduate School of Science, The University of Tokyo 7-3-1, Hongo Bunkyo Tokyo Japan 113-0033 Futamata H Fukuda M Umeda R Yamashita K Tomita A Takahashi S Shikakura T Hayashi S Kusakizako T Nishizawa T Homma K Nureki O 3.5 10.6019/EMPIAR-11199 EMDB molecule EMD-31757 EMD-31758 EMD-31759 Futamata H Fukuda M Umeda R Yamashita K Tomita A Takahashi S Shikakura T Hayashi S Kusakizako T Nishizawa T Homma K Nureki O Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility Nature communications Nat Commun 1 13 2022 English 10.1038/s41467-022-34017-x 36266333
This entry contains raw movies for three datasets of thermostabilized human prestin in complex with chloride, sulfate or salicylate.
 Cl bound /data/Cl_bound_structure/movies micrographs - multiframe TIFF TIFF 9361 64 2 64 UNSIGNED BYTE 5760 0.83 4092 0.83
HT: 300 kV Cs: 2.7 mm Flux: 0.84 e/Å2/frame Please discard the first frame, which was corrupted due to a problem with the CDS mode. Please use 'CountCDSRef_futamata1-3_3-A-001_0000_Feb22_11.46.30.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file names represent the direction of beam shift.
 Sulfate bound /data/SO4_bound_structure/movies micrographs - multiframe TIFF TIFF 8155 64 2 64 UNSIGNED BYTE 5760 0.83 4092 0.83
HT: 300 kV Cs: 2.7 mm Flux: 0.84 e/Å2/frame Please discard the first frame, which was corrupted due to a problem with the CDS mode. Please use 'CountCDSRef_futamata4-1_36-A-001_0000_Mar05_13.25.01.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file names represent the direction of beam shift.
 Salicylate bound /data/Salicylate_bound_structure/movies micrographs - multiframe TIFF TIFF 6751 64 2 64 UNSIGNED BYTE 5760 0.83 4092 0.83
HT: 300 kV Cs: 2.7 mm Flux: 0.84 e/Å2/frame Please discard the first frame, which was corrupted due to a problem with the CDS mode. Please use 'CountCDSRef_futamata3-2_69-6_0000_Mar03_17.09.30.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file names represent the direction of beam shift.