<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11210" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-09-06</depositionDate>
            <releaseDate>2022-10-10</releaseDate>
            <updateDate>2022-10-10</updateDate>
        </keyDates>
        <title>Cryo-EM structure of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae without inhibitors</title>
        <correspondingAuthor>
            <authorORCID>0000-0002-1792-554X</authorORCID>
            <firstName>Hideto</firstName>
            <lastName>Miyoshi</lastName>
            <organization type="academic">Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University</organization>
            <street>Kitashirakawa Oiwake, Sakyo</street>
            <townOrCity>Kyoto</townOrCity>
            <stateOrProvince>Kyoto</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>606-8502</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-1792-554X</authorORCID>
            <firstName>Hideto</firstName>
            <lastName>Miyoshi</lastName>
            <organization type="academic">Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University</organization>
            <street>Kitashirakawa Oiwakecho, Sakyo</street>
            <townOrCity>Kyoto</townOrCity>
            <stateOrProvince>Kyoto</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>606-8502</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3913-7330">Kishikawa J</author>
            <author authorORCID="0000-0002-8620-1801">Ishikawa M</author>
            <author authorORCID="0000-0003-4950-0527">Masuya T</author>
            <author authorORCID="0000-0001-6601-2854">Murai M</author>
            <author>Kitazumi Y</author>
            <author>Butler NL</author>
            <author authorORCID="0000-0002-8879-6685">Kato T</author>
            <author authorORCID="0000-0003-0708-3438">Barquera B</author>
            <author authorORCID="0000-0002-1792-554X">Miyoshi H</author>
        </authorsList>
        <datasetSize units="TB">2.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-11210</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-33242</emdbEntry>
            <emdbEntry>EMD-33243</emdbEntry>
            <emdbEntry>EMD-33244</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-3913-7330" order="1">Kishikawa J</author>
                    <author authorORCID="0000-0002-8620-1801" order="2">Ishikawa M</author>
                    <author authorORCID="0000-0003-4950-0527" order="3">Masuya T</author>
                    <author authorORCID="0000-0001-6601-2854" order="4">Murai M</author>
                    <author order="5">Kitazumi Y</author>
                    <author order="6">Butler NL</author>
                    <author authorORCID="0000-0002-8879-6685" order="7">Kato T</author>
                    <author authorORCID="0000-0003-0708-3438" order="8">Barquera B</author>
                    <author authorORCID="0000-0002-1792-554X" order="9">Miyoshi H</author>
                    <title>Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>13</volume>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-022-31718-1</externalReferences>
                    <externalReferences type="pubmed">35882843</externalReferences>
                    <details>This entry contains raw movies for structures EMD-33242 (Na+-NQR, state 1), EMD-33243 (Na+-NQR, state 2) and EMD-33244 (Na+-NQR, state 3).</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned, non-dose weighted movies of Na+-pumping NADH-ubiquinone oxidoreductase without inhibitors, states 1-3</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>9027</numImagesOrTiltSeries>
        <framesPerImage>59</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>59</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.88</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.88</pixelHeight>
        </dimensions>
        <details>HT: 300 kV
Cs-corrected 0.039 mm
65 e/Å2 over 59 frames = 1.1 e/Å2/frame

Please use CountCDSRef_20211015_solNQR_90_0000_Oct15_13.05.33.mrc as a gain reference without rotation and flipping.
The four digits (0000 to 0008) in the file names represent the direction of beam shift applied during data collection. We recommend beam tilt correction per directions.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
