REL 2022-09-16 2022-10-04 2022-10-04 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street San Francisco California United States 94143 0000-0001-5037-7322 Melinda M Diver Structural Biology Program, Memorial Sloan Kettering Cancer Center 430 East 67th Street New York New York United States 10065 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street San Francisco California United States 94143 0000-0002-6365-4867 David Julius Department of Physiology, University of California, San Francisco 600 16th Street San Francisco California United States 94143 Diver MM Cheng Y Julius D 129.7 10.6019/EMPIAR-11233 EMDB molecule EMD-0639 Diver MM Cheng Y Julius D Science Science United States 6460 365 1434 1440 2019 English 10.1126/science.aax6672 31488702

The transient receptor potential melastatin 8 (TRPM8) ion channel is the primary detector of environmental cold and an important target for treating pathological cold hypersensitivity. Here, we present cryo–electron microscopy structures of TRPM8 in ligand-free, antagonist-bound, or calcium-bound forms, revealing how robust conformational changes give rise to two nonconducting states, closed and desensitized.

Dataset of TRPM8 bound to calcium, intermediate processed stack /data/Intermediate_Particle_Stack picked particles - single frame - processed MRC MRC 297750 1 32 BIT FLOAT 320 1.059 320 1.059

This dataset contains TRPM8 particles bound to calcium. It is a larger particle stack containing ~300k particles, a subset of which yielded the final published reconstruction.

Dataset of TRPM8 bound to calcium, processed stack from final reconstruction /data/Final_Particle_Stack picked particles - single frame - processed MRC MRC 42040 1 32 BIT FLOAT 320 1.059 320 1.059

This dataset contains TRPM8 particles bound to calcium. It contains the ~40k particles that went into the final published reconstruction. It is a subset of the larger particle stack containing ~300k particles (uploaded in parallel).