REL 2022-10-03 2022-10-17 2022-10-17 Cryo-EM structure of non gastric H,K-ATPase alpha2 mutants 0000-0003-2681-5921 Kazuhiro Abe Cellular and Structural Physiology Institute, Nagoya University 1, Furo-cho, Chikusa-ku Nagoya Aichi Japan 464-8601 0000-0003-2681-5921 Kazuhiro Abe Cellular and Structural Physiology Institute, Nagoya University 1, Furo-cho, Chikusa-ku Nagoya Aichi Japan 464-8601 Abe K Nakanishi H 3.8 10.6019/EMPIAR-11248 EMDB molecule EMD-32954 EMD-32955 EMD-32956 EMD-32957 Young VC Nakanishi H Meyer DJ Nishizawa T Oshima A Artigas P Abe K Structure and function of H+/K+ pump mutants reveal Na+/K+ pump mechanism Nature Communications Nat Commun 13 2022 English 10.1038/s41467-022-32793-0 36085139
This entry contains four CryoEM datasets reported in the paper. - ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954 - ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955 - ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956 - ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957
 K3 movies for ngHKA K794A (K+)E2-Pi state, PDB 7X21, EMD-32954 /data/7x21/ micrographs - multiframe TIFF TIFF 3106 48 2 48 UNSIGNED BYTE 5760 variable 4092 variable
- 300 kV - Cs 2.7 mm - 0.83 Å/pix - 53 e-/Å2 in 50 frames Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode. Please use 'CountRef_abe3-1_5-015_0000_Sep08_13.42.21.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file name represent the beam shift directions.
 K3 movies for ngHKA K794S (2K+)E2-Pi state, PDB 7X22, EMD-32955 /data/7x22/ micrographs - multiframe TIFF TIFF 3141 48 2 48 UNSIGNED BYTE 5760 variable 4092 variable
- 300 kV - Cs 2.7 mm - 0.83 Å/pix - 53 e-/Å2 in 50 frames Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode. Please use 'CountRef_abe2-1_5-A-001_0000_Feb21_16.39.37.mrc' for files under 'Movies_2-1' and 'CountRef_abe2-1_3-A-001_0000_Feb21_23.51.48.mrc' for files under 'Movies_2-3' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file name represent the beam shift directions.
 K3 movies for ngHKA SPWC 3Na+ꞏE1-ATP state, PDB 7X23, EMD-32956 /data/7x23/ micrographs - multiframe TIFF TIFF 3627 48 2 48 UNSIGNED BYTE 5760 variable 4092 variable
- 300 kV - Cs 2.7 mm - 0.83 Å/pix - 53 e-/Å2 in 50 frames Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode. Please use 'CountCDSRef_abe1-1_21-A-001-1_0000_Aug02_14.03.28.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file name represent the beam shift directions.
 K3 movies for ngHKA SPWC (2K+)E2-Pi state, PDB 7X24, EMD-32957 /data/7x24/ micrographs - multiframe TIFF TIFF 4150 48 2 48 UNSIGNED BYTE 5760 variable 4092 variable
- 300 kV - Cs 2.7 mm - 0.83 Å/pix - 53 e-/Å2 in 50 frames Please exclude the first frame from processing because it is corrupted due to a bug in the CDS mode. Please use 'CountCDSRef_abe1-1_11_0000_Jun14_15.38.45.mrc' as a gain reference without rotation or flipping. Four digits (0000 to 0008) in the file name represent the beam shift directions.