<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11286" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-10-25</depositionDate>
            <releaseDate>2022-11-28</releaseDate>
            <updateDate>2022-11-28</updateDate>
        </keyDates>
        <title>Cryo-EM Structure of Formate Dehydrogenase 1 from Methylorubrum extorquens AM1</title>
        <correspondingAuthor>
            <authorORCID>0000-0001-9767-4922</authorORCID>
            <firstName>Keisei</firstName>
            <lastName>Sowa</lastName>
            <organization type="academic">Division of Applied Life Sciences, Graduate School of Agricul-ture, Kyoto University</organization>
            <street>Sakyo</street>
            <townOrCity>Kyoto</townOrCity>
            <stateOrProvince>Kyoto</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>606-8502</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0001-9767-4922</authorORCID>
            <firstName>Keisei</firstName>
            <lastName>Sowa</lastName>
            <organization type="academic">Division of Applied Life Sciences,  Graduate School of Agriculture,  Kyoto University,</organization>
            <street>Sakyo</street>
            <townOrCity>Kyoto</townOrCity>
            <stateOrProvince>kyoto</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>606-8502</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-3839-0713">Yoshikawa T</author>
            <author authorORCID="0000-0001-9512-7087">Makino F</author>
            <author authorORCID="0000-0002-5543-7459">Miyata T</author>
            <author authorORCID="0000-0001-8352-582X">Suzuki Y</author>
            <author authorORCID="0000-0003-2035-954X">Tanaka H</author>
            <author authorORCID="0000-0003-2911-5875">Namba K</author>
            <author authorORCID="0000-0002-4667-7020">Kano K</author>
            <author authorORCID="0000-0001-9767-4922">Sowa K</author>
            <author authorORCID="0000-0001-6171-6457">Kitazumi Y</author>
            <author authorORCID="0000-0003-1317-6243">Shirai O</author>
        </authorsList>
        <datasetSize units="TB">1.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-11286</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-32151</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-3839-0713" order="1">Yoshikawa T</author>
                    <author authorORCID="0000-0001-9512-7087" order="2">Makino F</author>
                    <author authorORCID="0000-0002-5543-7459" order="3">Miyata T</author>
                    <author authorORCID="0000-0001-8352-582X" order="4">Suzuki Y</author>
                    <author authorORCID="0000-0003-2035-954X" order="5">Tanaka H</author>
                    <author authorORCID="0000-0003-2911-5875" order="6">Namba K</author>
                    <author authorORCID="0000-0002-4667-7020" order="7">Kano K</author>
                    <author authorORCID="0000-0001-9767-4922" order="8">Sowa K</author>
                    <author authorORCID="0000-0001-6171-6457" order="9">Kitazumi Y</author>
                    <author authorORCID="0000-0003-1317-6243" order="10">Shirai O</author>
                    <title>Multiple electron transfer pathways of tungsten-containing formate dehydrogenase in direct electron transfer-type bioelectrocatalysis</title>
                    <journal>Chemical communications (Cambridge, England)</journal>
                    <journalAbbreviation>Chem Commun (Camb)</journalAbbreviation>
                    <country></country>
                    <issue>45</issue>
                    <volume>58</volume>
                    <firstPage>6478</firstPage>
                    <lastPage>6481</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1039/d2cc01541b</externalReferences>
                    <externalReferences type="pubmed">35535582</externalReferences>
                    <details>This deposition contains unaligned raw movies.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Raw movies</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7650</numImagesOrTiltSeries>
        <framesPerImage>40</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>40</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.87</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.87</pixelHeight>
        </dimensions>
        <details>300 kV
Cs 2.7 mm
2.0 e/Å2/frame

Note that the pixel size in the image header (0.767 Å/px) is wrong. 0.87 Å/px is the calibrated value.

Please use CountCDSRef_FoDH_5mg_Au_45-2_000.mrc as a gain reference without rotation or flipping.

The last three digits (000 to 074) in the movie file names represent the beam shift directions: 5 x 5 (holes) x 3 (images per hole). The preceding digits (e.g. 48-22) are the stage positions.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
