REL 2022-11-14 2023-07-18 2023-07-18 Purified tails from bacteriophage T5 0000-0001-9226-3382 Romain Linares Institut de Biologie Structurale 71 avenue des martyrs GRENOBLE - France 38044 CEDEX9 0000-0001-9226-3382 Romain Linares Institut de Biologie Structurale 71 avenue des Martyrs GRENOBLE France 38044 CEDEX 9 Linares R Arnaud CA Effantin G Darnault C Epalle NH Boeri Erba E Schoehn G Breyton C 6.6 10.6019/EMPIAR-11319 EMDB virus EMD-13953 EMD-14733 EMD-14790 EMD-14869 Linares R Arnaud CA Effantin G Darnault C Epalle NH Boeri Erba E Schoehn G Breyton C Structural basis of bacteriophage T5 infection trigger and <i>E. coli</i> cell wall perforation Science advances Sci Adv 12 9 2023 English 10.1126/sciadv.ade9674 36961893
This is a cryo-EM sample of purified tails from bacteriophage T5. Typically, 3.5 µL of T5 tail sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0) The dataset contains 3208 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.
 Linares R Arnaud CA Effantin G Darnault C Epalle NH Boeri Erba E Schoehn G Breyton C Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation BioRxiv 10.1101/2022.09.20.507954
This is a cryo-EM sample of purified tails from bacteriophage T5. Typically, 3.5 µL of T5 tail sample were deposited on a freshly glow discharged (25 mA, 30 sec) Cu/Rh 300 mesh Quantifoil R 2/1 EM grids and plunge frozen in nitrogen-cooled liquid ethane using a ThermoFisher Mark IV Vitrobot device (100% humidity, 20°C, 5s blotting time, blot force 0) The dataset contains 3208 40-frame movie, acquired on a Thermo Fisher Scientific Titan Krios G3 TEM (European Synchrotron Radiation Facility, Grenoble, France) operated at 300 kV and equipped with a Gatan Quantum energy filter coupled to a Gatan K2 summit direct electron detector. Automated data collection was performed using Thermo Fisher Scientific EPU software, with a typical defocus range of -1.0 to -3.0 µm and a total dose of 40 e-/Å2 per movie. A nominal magnification of 105.000x was used, resulting in a calibrated pixel size at the specimen level of 1.351 Å.
 Unaligned multi-frame micrographs of phage T5 tails /data micrographs - multiframe MRC MRC 3208 40 32 BIT FLOAT 3838 1.351 3710 1.351