<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11351" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2022-12-07</depositionDate>
            <releaseDate>2023-01-16</releaseDate>
            <updateDate>2023-01-16</updateDate>
        </keyDates>
        <title>Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-1813-7008</authorORCID>
            <firstName>Osamu</firstName>
            <lastName>Nureki</lastName>
            <organization type="academic">Department of Biological Sciences, Graduate School of Science, The University of Tokyo</organization>
            <street>7-3-1, Hongo</street>
            <townOrCity>Bunkyo</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0003-1813-7008</authorORCID>
            <firstName>Osamu</firstName>
            <lastName>Nureki</lastName>
            <organization type="academic">Department of Biological Sciences, Graduate School of Science, The University of Tokyo</organization>
            <street>7-3-1, Hongo</street>
            <townOrCity>Bunkyo</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>113-0033</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Nagiri C</author>
            <author authorORCID="0000-0002-3366-3586">Kobayashi K</author>
            <author authorORCID="0000-0003-3887-1429">Tomita A</author>
            <author>Kato M</author>
            <author authorORCID="0000-0002-5442-7582">Yamashita K</author>
            <author>Nishizawa T</author>
            <author>Inoue A</author>
            <author authorORCID="0000-0003-4813-5740">Shihoya W</author>
            <author authorORCID="0000-0003-1813-7008">Nureki O</author>
        </authorsList>
        <datasetSize units="TB">2.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-11351</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-33227</emdbEntry>
            <emdbEntry>EMD-33228</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Nureki I</author>
                    <author authorORCID="0000-0002-3366-3586" order="2">Kobayashi K</author>
                    <author authorORCID="0000-0001-5728-7573" order="3">Tanaka T</author>
                    <author authorORCID="0000-0001-5034-3819" order="4">Demura K</author>
                    <author order="5">Inoue A</author>
                    <author authorORCID="0000-0003-4813-5740" order="6">Shihoya W</author>
                    <author authorORCID="0000-0003-1813-7008" order="7">Nureki O</author>
                    <title>Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol</title>
                    <journal>Biochemical and biophysical research communications</journal>
                    <journalAbbreviation>Biochem Biophys Res Commun</journalAbbreviation>
                    <country></country>
                    <volume>611</volume>
                    <firstPage>158</firstPage>
                    <lastPage>164</lastPage>
                    <year>2022</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.bbrc.2022.04.065</externalReferences>
                    <externalReferences type="pubmed">35489202</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned, non-dose weighted raw movies for B3AR with isoproterenol (EMD-33227)</name>
        <directory>/data/20210409_Nureki_ikko2_CDS_data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3789</numImagesOrTiltSeries>
        <framesPerImage>64</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>64</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- 300 kV
- Cs 2.7 mm
- 50 e/Å2 over 64 frames

Please use "20210409_Nureki_ikko2_CDS_data/CountCDSRef_ikko2_82-1_0000_Apr09_14.47.39.mrc" as a gain reference without rotation or flipping.
Please exclude the first frame in each movie, which was corrupted due to a bug in the CDS mode.

Four digits (0000 to 0008) in the file names represent the direction of beam shift.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned, non-dose weighted raw movies for B3AR with solabegron (EMD-33228)</name>
        <directory>/data/20210113_Nureki_Ikko1-3_data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>4833</numImagesOrTiltSeries>
        <framesPerImage>64</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>64</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>- 300 kV
- Cs 2.7 mm
- 50 e/Å2 over 64 frames

Please use "20210113_Nureki_Ikko1-3_data/CountCDSRef_ikko1-3_47-1_0000_Jan13_18.08.04.mrc" as a gain reference without rotation or flipping.
Please exclude the first frame in each movie, which was corrupted due to a bug in the CDS mode.

Four digits (0000 to 0008) in the file names represent the direction of beam shift.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
