SBF-SEM micrographs of A. algerae microsporidia spores, 45 min germination

REL 2023-01-06 2023-10-13 2023-10-13 SBF-SEM micrographs of A. algerae microsporidia spores, 45 min germination 0000-0003-0624-6178 Gira Bhabha NYU School of Medicine New York City NY United States 10016 0000-0003-0624-6178 Gira Bhabha NYU School of Medicine New York City NY United States 10016 0000-0002-2570-0404 Damian Ekiert NYU School of Medicine New York City NY United States 10016 Davydov A Jaroenlak P Ekiert D Bhabha G 55.9 10.6019/EMPIAR-11368 SBF-SEM cell Chang R Davydov A Jaroenlak P Budaitis B Ekiert D Bhabha G Prakash M Energetics of the Microsporidian Polar Tube Invasion Machinery ELife Elife United States 2023 English 10.7554/eLife.86638.1 SBF-SEM micrographs of A. algerae microsporidia spores, 45 min germination /data micrographs - single frame TIFF TIFF 300 1 UNSIGNED 16 BIT INTEGER 10000 2.2 10000 2.2

Micrographs imaged using the Gatan OnPoint BSE detector in a Zeiss Gemini 300 VP FESEM equipped with a Gatan 3View automatic microtome. The system was set to cut 40 nm slices, imaged with gas injection setting at 40% (2.9*10^-3 mBar) with Focus Charge Compensation to reduce electron accumulation charging artifacts. Images were recorded after each round of sectioning from the blockface using the SEM beam at 1.5 keV with a beam aperture size of 30 μm and a dwell time of 2.0 μsec/pixel.